Hairpin Plasmid--a Novel Linear DNA of Perfect Hairpin Structure

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 Jul 1

PMID 2992950

Citations 17

Authors

Y Kikuchi

K Hirai

N Gunge

F Hishinuma

Affiliations

Soon will be listed here.

Abstract

The terminal structures of deletion derivatives of linear DNA killer plasmid from yeast were analyzed. The yeast Kluyveromyces lactis harbors two unique double-stranded linear DNA killer plasmids, pGKL1 of 8.9 kb and pGKL2 of 13.4 kb. The killer toxin and the resistance to the killer are coded by pGKL1, while pGKL2 is required for the maintenance of pGKL1 in the cell. When the pGKL plasmids from K. lactis were transferred into Saccharomyces cerevisiae by transformation, non-killer transformants harboring pGKL2 and new plasmids, F1 of 7.8 kb and F2 of 3.9 kb, were obtained. F2 was shown to be a linear DNA arising from a 5-kb deletion of the right part of pGKL1. F1 was an inverted dimer of F2. Here we show that F2 has two different terminal structures: one end has a protein attached at the 5' terminus whereas the two strands of duplex are linked together at the other end, thus forming a hairpin structure. This is a novel type of autonomously replicating DNA molecule.

Citing Articles

Linear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation.

Perez M, Angelov A, Ubelacker M, Torres Tejerizo G, Farias M, Liebl W Environ Microbiol. 2025; 27(1):e70020.

PMID: 39777960 PMC: 11707576. DOI: 10.1111/1462-2920.70020.

Origin-Dependent Inverted-Repeat Amplification: Tests of a Model for Inverted DNA Amplification.

Brewer B, Payen C, Di Rienzi S, Higgins M, Ong G, Dunham M PLoS Genet. 2015; 11(12):e1005699.

PMID: 26700858 PMC: 4689423. DOI: 10.1371/journal.pgen.1005699.

Strategies and approaches in plasmidome studies-uncovering plasmid diversity disregarding of linear elements?.

Dib J, Wagenknecht M, Farias M, Meinhardt F Front Microbiol. 2015; 6:463.

PMID: 26074886 PMC: 4443254. DOI: 10.3389/fmicb.2015.00463.

Long palindromes formed in Streptomyces by nonrecombinational intra-strand annealing.

Qin Z, Cohen S Genes Dev. 2000; 14(14):1789-96.

PMID: 10898793 PMC: 316786.

Hairpin and dimer structures of linear plasmid-like DNAs in mitochondria of Paramecium caudatum.

Endoh H, Yazaki K, Takahashi M, Tsukii Y Curr Genet. 1994; 27(1):90-4.

PMID: 7750152 DOI: 10.1007/BF00326584.

References

Kikuchi Y, Hirai K, Hishinuma F . The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins. Nucleic Acids Res. 1984; 12(14):5685-92. PMC: 320023. DOI: 10.1093/nar/12.14.5685. View

Desiderio S, Kelly Jr T . Structure of the linkage between adenovirus DNA and the 55,000 molecular weight terminal protein. J Mol Biol. 1981; 145(2):319-37. DOI: 10.1016/0022-2836(81)90208-4. View

Kemble R, Thompson R . S1 and S2, the linear mitochondrial DNAs present in a male sterile line of maize, possess terminally attached proteins. Nucleic Acids Res. 1982; 10(24):8181-90. PMC: 327078. DOI: 10.1093/nar/10.24.8181. View

Sugisaki Y, Gunge N, Sakaguchi K, Yamasaki M, Tamura G . Characterization of a novel killer toxin encoded by a double-stranded linear DNA plasmid of Kluyveromyces lactis. Eur J Biochem. 1984; 141(2):241-5. DOI: 10.1111/j.1432-1033.1984.tb08183.x. View

Pring D, Levings C, Hu W, Timothy D . Unique DNA associated with mitochondria in the "S"-type cytoplasm of male-sterile maize. Proc Natl Acad Sci U S A. 1977; 74(7):2904-8. PMC: 431340. DOI: 10.1073/pnas.74.7.2904. View

Challberg M, Kelly T . Eukaryotic DNA replication: viral and plasmid model systems. Annu Rev Biochem. 1982; 51:901-34. DOI: 10.1146/annurev.bi.51.070182.004345. View

Silberklang M, Gillum A, RajBhandary U . Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs. Methods Enzymol. 1979; 59:58-109. DOI: 10.1016/0076-6879(79)59072-7. View

Inciarte M, Salas M, Sogo J . Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29. J Virol. 1980; 34(1):187-99. PMC: 288685. DOI: 10.1128/JVI.34.1.187-199.1980. View

Hishinuma F, Nakamura K, Hirai K, Nishizawa R, Gunge N, Maeda T . Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast. Nucleic Acids Res. 1984; 12(19):7581-97. PMC: 320182. DOI: 10.1093/nar/12.19.7581. View

10.

Sugisaki Y, Gunge N, Sakaguchi K, Yamasaki M, Tamura G . Kluyveromyces lactis killer toxin inhibits adenylate cyclase of sensitive yeast cells. Nature. 1983; 304(5925):464-6. DOI: 10.1038/304464a0. View

11.

Salas M, Mellado R, Vinuela E . Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29. J Mol Biol. 1978; 119(2):269-91. DOI: 10.1016/0022-2836(78)90438-2. View

12.

Rekosh D, Russell W, Bellet A, Robinson A . Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977; 11(2):283-95. DOI: 10.1016/0092-8674(77)90045-9. View

13.

Arrand J, Roberts R . The nucleotide sequences at the termini of adenovirus-2 DNA. J Mol Biol. 1979; 128(4):577-94. DOI: 10.1016/0022-2836(79)90294-8. View

14.

Steenbergh P, Maat J, Van Ormondt H, Sussenbach J . The nucleotide sequence at the termini of adenovirus type 5 DNA. Nucleic Acids Res. 1977; 4(12):4371-89. PMC: 343247. DOI: 10.1093/nar/4.12.4371. View

15.

Gunge N, Murata K, Sakaguchi K . Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis. J Bacteriol. 1982; 151(1):462-4. PMC: 220260. DOI: 10.1128/jb.151.1.462-464.1982. View

16.

Sor F, Wesolowski M, Fukuhara H . Inverted terminal repetitions of the two linear DNA associated with the killer character of the yeast Kluyveromyces lactis. Nucleic Acids Res. 1983; 11(15):5037-44. PMC: 326235. DOI: 10.1093/nar/11.15.5037. View

17.

Hirochika H, Nakamura K, Sakaguchi K . A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs. EMBO J. 1984; 3(4):761-6. PMC: 557423. DOI: 10.1002/j.1460-2075.1984.tb01881.x. View

18.

Yehle C . Genome-linked protein associated with the 5' termini of bacteriophage phi29 DNA. J Virol. 1978; 27(3):776-83. PMC: 525865. DOI: 10.1128/JVI.27.3.776-783.1978. View

19.

Niwa O, Sakaguchi K, Gunge N . Curing of the killer deoxyribonucleic acid plasmids of Kluyveromyces lactis. J Bacteriol. 1981; 148(3):988-90. PMC: 216303. DOI: 10.1128/jb.148.3.988-990.1981. View

20.

Harding N, Ito J . DNA replication of bacteriophage phi 29: characterization of the intermediates and location of the termini of replication. Virology. 1980; 104(2):323-38. DOI: 10.1016/0042-6822(80)90337-2. View