Evidence for IFN-beta Heterogeneity in a Substrain of Namalwa Cells

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 1985 Apr 1

PMID 2992372

Citations 1

Authors

H Borg

I Nordensson

T Ny

E Lundgren

Affiliations

Soon will be listed here.

Abstract

A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule.

Citing Articles

Repression of beta interferon gene expression in virus-infected cells is correlated with a poly(A) tail elongation.

Dehlin E, von Gabain A, Alm G, Dingelmaier R, Resnekov O Mol Cell Biol. 1996; 16(2):468-74.

PMID: 8552072 PMC: 231023. DOI: 10.1128/MCB.16.2.468.

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