A Role of a Newly Identified Isomerase From in Erythritol Catabolism

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2018 Jun 19

PMID 29910781

Citations 11

Authors

Aleksandra M Mironczuk

Anna Biegalska

Karolina Zugaj

Dorota A Rzechonek

Adam Dobrowolski

Affiliations

Soon will be listed here.

Abstract

Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast . Despite the recent identification of the transcription factor of erythritol utilization (), the metabolic pathway of erythritol catabolism remains unknown. In this study we identified a new gene, , involved in erythritol assimilation. analysis showed that is a putative isomerase and it is localized in the same region as . qRT-PCR analysis of showed a significant increase in g expression during growth on erythritol and after overexpression of . Moreover, the deletion strain showed significantly impaired erythritol assimilation, whereas synthesis of erythritol remained unchanged. The results showed that is involved in erythritol assimilation; thus we named the gene . Moreover, we suggest the metabolic pathway of erythritol assimilation in yeast .

Citing Articles

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