DnaA Protein-regulated Transcription: Effects on the in Vitro Replication of Escherichia Coli Minichromosomes

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 Feb 1

PMID 2990902

Citations 53

Authors

H Lother

R Kolling

C Kucherer

M Schauzu

Affiliations

Soon will be listed here.

Abstract

Both initiation of replication and initiation of transcription are influenced by dnaA protein, when minichromosomes are assayed in vitro for dnaA protein complementation. This dnaA protein effect is seen only if minichromosomes are used containing the 16-kd promoter, from which transcription is directed into the minimal origin. Determination of the 16-kd promoter activity both in vivo and in vitro showed that this strong promoter is specifically repressed by dnaA protein. The 16-kd promoter is thus an integral regulatory region of oriC.

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