RecBCD Coordinates Repair of Two Ends at a DNA Double-strand Break, Preventing Aberrant Chromosome Amplification

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2018 Jun 15

PMID 29901759

Citations 8

Authors

Martin A White

Benura Azeroglu

Manuel A Lopez-Vernaza

A M Mahedi Hasan

David R F Leach

Affiliations

Soon will be listed here.

Abstract

DNA double-strand break (DSB) repair is critical for cell survival. A diverse range of organisms from bacteria to humans rely on homologous recombination for accurate DSB repair. This requires both coordinate action of the two ends of a DSB and stringent control of the resultant DNA replication to prevent unwarranted DNA amplification and aneuploidy. In Escherichia coli, RecBCD enzyme is responsible for the initial steps of homologous recombination. Previous work has revealed recD mutants to be nuclease defective but recombination proficient. Despite this proficiency, we show here that a recD null mutant is defective for the repair of a two-ended DSB and that this defect is associated with unregulated chromosome amplification and defective chromosome segregation. Our results demonstrate that RecBCD plays an important role in avoiding this amplification by coordinating the two recombining ends in a manner that prevents divergent replication forks progressing away from the DSB site.

Citing Articles

RecBCD enzyme: mechanistic insights from mutants of a complex helicase-nuclease.

Amundsen S, Smith G Microbiol Mol Biol Rev. 2023; 87(4):e0004123.

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Specific targeting of plasmids with Argonaute enables genome editing.

Esyunina D, Okhtienko A, Olina A, Panteleev V, Prostova M, Aravin A Nucleic Acids Res. 2023; 51(8):4086-4099.

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Role for DNA double strand end-resection activity of RecBCD in control of aberrant chromosomal replication initiation in Escherichia coli.

Goswami S, Gowrishankar J Nucleic Acids Res. 2022; 50(15):8643-8657.

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Essential Role for an Isoform of Escherichia coli Translation Initiation Factor IF2 in Repair of Two-Ended DNA Double-Strand Breaks.

Mallikarjun J, Gowrishankar J J Bacteriol. 2022; 204(4):e0057121.

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DNA targeting and interference by a bacterial Argonaute nuclease.

Kuzmenko A, Oguienko A, Esyunina D, Yudin D, Petrova M, Kudinova A Nature. 2020; 587(7835):632-637.

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