Lymph Node γδ and αβ CD8 T Cells Share Migratory Properties

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jun 14

PMID 29895956

Citations 9

Authors

Milas Ugur

Anne Kaminski

Oliver Pabst

Affiliations

Soon will be listed here.

Abstract

During immune responses, T cells differentiate into subsets with different functions and migratory properties. Here we characterize migratory behavior of endogenous αβ CD8 and γδ T cells in lymph nodes by long-term tracking following in vivo photoconversion. We identified subsets of γδ T cells with distinct circulation kinetics that closely mirrored migratory subsets of αβ CD8 T cells. Notably, αβ CD8 and γδ T cells both comprised resident populations which stayed in lymph nodes for 4 weeks without circulation or proliferation. Furthermore, in contrast to the common conception, we observed that central memory αβ CD8 T cells circulate with slower kinetics than naïve cells. Our results show that, similar to αβ T cells, γδ T cells can acquire distinct migratory properties during their development and differentiation and reveal unexpected intricacies of T cell migratory patterns.

Citing Articles

Circulating NK cells establish tissue residency upon acute infection of skin and mediate accelerated effector responses to secondary infection.

Torcellan T, Friedrich C, Doucet-Ladeveze R, Ossner T, Sole V, Riedmann S Immunity. 2023; 57(1):124-140.e7.

PMID: 38157853 PMC: 10783803. DOI: 10.1016/j.immuni.2023.11.018.

Same yet different - how lymph node heterogeneity affects immune responses.

Cruz de Casas P, Knopper K, Sarkar R, Kastenmuller W Nat Rev Immunol. 2023; 24(5):358-374.

PMID: 38097778 DOI: 10.1038/s41577-023-00965-8.

Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks.

Ugur M, Labios R, Fenton C, Knopper K, Jobin K, Imdahl F Immunity. 2023; 56(8):1778-1793.e10.

PMID: 37463581 PMC: 10433941. DOI: 10.1016/j.immuni.2023.06.020.

MHCII expression on gut macrophages supports T cell homeostasis and is regulated by microbiota and ontogeny.

Guillaume J, Leufgen A, Hager F, Pabst O, Cerovic V Sci Rep. 2023; 13(1):1509.

PMID: 36707699 PMC: 9883227. DOI: 10.1038/s41598-023-28554-8.

PD-1 and TIM-3 differentially regulate subsets of mouse IL-17A-producing γδ T cells.

Edwards S, Hedley A, Hoevenaar W, Wiesheu R, Glauner T, Kilbey A J Exp Med. 2022; 220(2).

PMID: 36480166 PMC: 9732671. DOI: 10.1084/jem.20211431.

References

Hayday A, Tigelaar R . Immunoregulation in the tissues by gammadelta T cells. Nat Rev Immunol. 2003; 3(3):233-42. DOI: 10.1038/nri1030. View

Chennupati V, Worbs T, Liu X, Malinarich F, Schmitz S, Haas J . Intra- and intercompartmental movement of gammadelta T cells: intestinal intraepithelial and peripheral gammadelta T cells represent exclusive nonoverlapping populations with distinct migration characteristics. J Immunol. 2010; 185(9):5160-8. DOI: 10.4049/jimmunol.1001652. View

Romagnoli P, Sheridan B, Pham Q, Lefrancois L, Khanna K . IL-17A-producing resident memory γδ T cells orchestrate the innate immune response to secondary oral Listeria monocytogenes infection. Proc Natl Acad Sci U S A. 2016; 113(30):8502-7. PMC: 4968747. DOI: 10.1073/pnas.1600713113. View

Osborn J, Mooster J, Hobbs S, Munks M, Barry C, Harty J . Enzymatic synthesis of core 2 O-glycans governs the tissue-trafficking potential of memory CD8 T cells. Sci Immunol. 2017; 2(16). PMC: 5786265. DOI: 10.1126/sciimmunol.aan6049. View

Gerlach C, Moseman E, Loughhead S, Alvarez D, Zwijnenburg A, Waanders L . The Chemokine Receptor CX3CR1 Defines Three Antigen-Experienced CD8 T Cell Subsets with Distinct Roles in Immune Surveillance and Homeostasis. Immunity. 2016; 45(6):1270-1284. PMC: 5177508. DOI: 10.1016/j.immuni.2016.10.018. View

Zeng X, Meyer C, Huang J, Newell E, Kidd B, Wei Y . Gamma delta T cells recognize haptens and mount a hapten-specific response. Elife. 2014; 3:e03609. PMC: 4174581. DOI: 10.7554/eLife.03609. View

Bonneville M, OBrien R, Born W . Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 2010; 10(7):467-78. DOI: 10.1038/nri2781. View

Cyster J, Schwab S . Sphingosine-1-phosphate and lymphocyte egress from lymphoid organs. Annu Rev Immunol. 2011; 30:69-94. DOI: 10.1146/annurev-immunol-020711-075011. View

Durand A, Audemard-Verger A, Guichard V, Mattiuz R, Delpoux A, Hamon P . Profiling the lymphoid-resident T cell pool reveals modulation by age and microbiota. Nat Commun. 2018; 9(1):68. PMC: 5754350. DOI: 10.1038/s41467-017-02458-4. View

10.

Cucak H, Yrlid U, Reizis B, Kalinke U, Johansson-Lindbom B . Type I interferon signaling in dendritic cells stimulates the development of lymph-node-resident T follicular helper cells. Immunity. 2009; 31(3):491-501. DOI: 10.1016/j.immuni.2009.07.005. View

11.

Mohammed R, Watson H, Vigar M, Ohme J, Thomson A, Humphreys I . L-selectin Is Essential for Delivery of Activated CD8(+) T Cells to Virus-Infected Organs for Protective Immunity. Cell Rep. 2016; 14(4):760-771. PMC: 4742564. DOI: 10.1016/j.celrep.2015.12.090. View

12.

von Andrian U, Mempel T . Homing and cellular traffic in lymph nodes. Nat Rev Immunol. 2003; 3(11):867-78. DOI: 10.1038/nri1222. View

13.

Zhang Y, Roth T, Gray E, Chen H, Rodda L, Liang Y . Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node. Elife. 2016; 5. PMC: 5017864. DOI: 10.7554/eLife.18156. View

14.

Shaner N, Patterson G, Davidson M . Advances in fluorescent protein technology. J Cell Sci. 2007; 120(Pt 24):4247-60. DOI: 10.1242/jcs.005801. View

15.

Morton A, Sefik E, Upadhyay R, Weissleder R, Benoist C, Mathis D . Endoscopic photoconversion reveals unexpectedly broad leukocyte trafficking to and from the gut. Proc Natl Acad Sci U S A. 2014; 111(18):6696-701. PMC: 4020091. DOI: 10.1073/pnas.1405634111. View

16.

Ogilvy S, Metcalf D, Gibson L, Bath M, Harris A, Adams J . Promoter elements of vav drive transgene expression in vivo throughout the hematopoietic compartment. Blood. 1999; 94(6):1855-63. View

17.

Vermijlen D, Prinz I . Ontogeny of Innate T Lymphocytes - Some Innate Lymphocytes are More Innate than Others. Front Immunol. 2014; 5:486. PMC: 4193329. DOI: 10.3389/fimmu.2014.00486. View

18.

Sheridan B, Romagnoli P, Pham Q, Fu H, Alonzo 3rd F, Schubert W . γδ T cells exhibit multifunctional and protective memory in intestinal tissues. Immunity. 2013; 39(1):184-95. PMC: 3749916. DOI: 10.1016/j.immuni.2013.06.015. View

19.

Schwab S, Cyster J . Finding a way out: lymphocyte egress from lymphoid organs. Nat Immunol. 2007; 8(12):1295-301. DOI: 10.1038/ni1545. View

20.

Fazilleau N, Eisenbraun M, Malherbe L, Ebright J, Pogue-Caley R, McHeyzer-Williams L . Lymphoid reservoirs of antigen-specific memory T helper cells. Nat Immunol. 2007; 8(7):753-61. DOI: 10.1038/ni1472. View