Human Serum Amyloid P Component. CDNA Isolation, Complete Sequence of Pre-serum Amyloid P Component, and Localization of the Gene to Chromosome 1

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 1985 Jun 25

PMID 2987268

Citations 21

Authors

E C Mantzouranis

S B Dowton

A S WHITEHEAD

M D Edge

G A BRUNS

H R Colten

Affiliations

Soon will be listed here.

Abstract

Complementary DNA clones corresponding to the human serum amyloid P component (SAP) were isolated, and the complete nucleotide and derived amino acid sequence of preSAP was determined. PreSAP biosynthesis is directed by a 1.1-kilobase mRNA. Synthesis and postsynthetic processing of preSAP in Xenopus oocytes result in secretion of a protein with mobility similar to native purified SAP when analyzed by sodium dodecyl sulfate gel electrophoresis. The human SAP gene is on chromosome 1, probably closely linked to the gene for C-reactive protein which encodes the related acute phase reactant found in human plasma.

Citing Articles

Human serum amyloid P component is an invariant constituent of amyloid deposits and has a uniquely homogeneous glycostructure.

Pepys M, Rademacher T, Amatayakul-Chantler S, Williams P, NOBLE G, Hutchinson W Proc Natl Acad Sci U S A. 1994; 91(12):5602-6.

PMID: 8202534 PMC: 44044. DOI: 10.1073/pnas.91.12.5602.

Mouse serum amyloid P-component (SAP) levels controlled by a locus on chromosome 1.

Mortensen R, Le P, Taylor B Immunogenetics. 1985; 22(4):367-75.

PMID: 4054960 DOI: 10.1007/BF00430920.

Isolation and characterization of guinea-pig serum amyloid P component.

Maudsley S, Hind C, Munn E, Buttress N, Pepys M Immunology. 1986; 59(2):317-22.

PMID: 3770806 PMC: 1453179.

The human C-reactive protein gene (CRP) and serum amyloid P component gene (APCS) are located on the proximal long arm of chromosome 1.

WHITEHEAD A, Colten H, Francke U Immunogenetics. 1986; 24(3):171-6.

PMID: 3759147 DOI: 10.1007/BF00364745.

The complete nucleotide and deduced amino acid sequence of mouse serum amyloid P component.

Ishikawa N, Shigemoto K, Maruyama N Nucleic Acids Res. 1987; 15(17):7186.

PMID: 3658681 PMC: 306210. DOI: 10.1093/nar/15.17.7186.