Alterations of ATP-sensitive K Channels in Human Umbilical Arterial Smooth Muscle During Gestational Diabetes Mellitus

Overview

Journal Pflugers Arch

Specialty Physiology

Date 2018 Jun 2

PMID 29855712

Citations 6

Authors

Hongliang Li

Sung Eun Shin

Mi Seon Seo

Jin Ryeol An

Kwon-Soo Ha

Eun-Taek Han

Seok-Ho Hong

Jeeyoung Kim

Mi-Jin Yim

Jeong Min Lee

Tae Gyu An

Jihan Jeon

Se Jin Lee

Sung Hun Na

Won Sun Park

Affiliations

Soon will be listed here.

Abstract

We investigated the alterations of ATP-sensitive K (K) channels in human umbilical arterial smooth muscle cells during gestational diabetes mellitus (GDM). The amplitude of the K current induced by application of the K channel opener pinacidil (10 μM) was reduced in the GDM group than in the control group. Pinacidil-induced vasorelaxation was also predominant in the normal group compared with the GDM group. Reverse transcription polymerase chain reaction and Western blot analysis suggested that the expression of K channel subunits such as Kir6.1, Kir6.2, and SUR2B were decreased in the GDM group relative to the normal group. The application of forskolin and adenosine, which activates protein kinase A (PKA) and thereby K channels, elicited K current in both the normal and GDM groups. However, the current amplitudes were not different between the normal and GDM groups. In addition, the expression levels of PKA subunits were not altered between the two groups. These results suggest that the reduction of K current and K channel-induced vasorelaxation are due to the decreased expression of K channels, not to the impairment of K-related signaling pathways.

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