Sodium Load and High Affinity Ouabain Binding in Rat and Guinea-pig Cardiac Tissue

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1985 Mar 1

PMID 2985161

Citations 8

Authors

S Herzig

K Mohr

Affiliations

Soon will be listed here.

Abstract

An estimation of the actual Na/K-ATPase transport activity in intact cardiac cells was made by measuring the binding of [3H]-ouabain to rat and guinea-pig ventricular strips. At the low [3H]-ouabain concentration of 1 nM equilibrium binding was hardly obtained after an incubation time of five hours. Different procedures known to alter the sodium load of the cardiac preparations influenced [3H]-ouabain binding: the sodium ionophore monensin enhanced [3H]-ouabain binding, the local anaesthetic dibucaine and a reduction of external sodium ion concentration diminished [3H]-ouabain binding; [3H]-ouabain binding was similarly affected by these procedures in the rat and guinea-pig. Since [3H]-ouabain binding occurred predominantly at the high-affinity binding sites of rat myocardium under the applied experimental conditions, it was concluded that these binding sites represent Na/K-ATPase molecules involved in sodium ion transport.

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References

Forssmann W, Girardier L . A study of the T system in rat heart. J Cell Biol. 1970; 44(1):1-19. PMC: 2107783. DOI: 10.1083/jcb.44.1.1. View

Dutta S, MARKS B . Factors that regulate ouabain-H3 accumulation by the isolated guinea-pig heart. J Pharmacol Exp Ther. 1969; 170(2):318-25. View

Bentfeld M, LULLMANN H, Peters T, Proppe D . Interdependence of ion transport and the action of quabain in heart muscle. Br J Pharmacol. 1977; 61(1):19-27. PMC: 1667636. DOI: 10.1111/j.1476-5381.1977.tb09735.x. View

Yamamoto S, Akera T, BRODY T . Sodium influx rate and ouabain-sensitive rubidium uptake in isolated guinea pig atria. Biochim Biophys Acta. 1979; 555(2):270-84. DOI: 10.1016/0005-2736(79)90167-6. View

Erdmann E, PHILIPP G, Scholz H . Cardiac glycoside receptor, (Na+ + K+)-ATPase activity and force of contraction in rat heart. Biochem Pharmacol. 1980; 29(24):3219-29. DOI: 10.1016/0006-2952(80)90295-6. View

Adams R, Schwartz A, GRUPP G, GRUPP I, Lee S, Wallick E . High-affinity ouabain binding site and low-dose positive inotropic effect in rat myocardium. Nature. 1982; 296(5853):167-9. DOI: 10.1038/296167a0. View

Alsen C, Peters T, SCHEUFLER E . Studies on the mechanism of the positive inotropic effect of ATX II (Anemonia sulcata) on isolated guinea pig atria. J Cardiovasc Pharmacol. 1982; 4(1):63-9. DOI: 10.1097/00005344-198201000-00011. View

Mansier P, Lelievre L . CA2+-free perfusion of rat heart reveals a (Na+ + K+)ATPase form highly sensitive to ouabain. Nature. 1982; 300(5892):535-7. DOI: 10.1038/300535a0. View

Arletti R, Bazzani C . Further studies on the frequency-dependent inotropic effect of ouabain on mammalian cardiac muscle. Pharmacol Res Commun. 1982; 14(8):725-30. DOI: 10.1016/s0031-6989(82)80078-7. View

10.

Temma K, Akera T . Decreases in active sodium pumping sites and their interaction with ouabain caused by low Na+ incubation of isolated guinea-pig atrial muscle. J Pharmacol Exp Ther. 1983; 225(3):660-6. View

11.

Kennedy R, Akera T, BRODY T . How increased sodium influx enhances digoxin-induced arrhythmias in guinea-pig atrial muscle. Eur J Pharmacol. 1983; 89(3-4):199-209. DOI: 10.1016/0014-2999(83)90495-8. View

12.

GRUPP G, Depover A, GRUPP I, Schwartz A . Analysis of the inotropic action of ouabain in rat ventricles: two apparent ouabain inotropic responses. Proc Soc Exp Biol Med. 1984; 175(1):39-43. DOI: 10.3181/00379727-175-41763. View

13.

Noel F, GODFRAIND T . Heterogeneity of ouabain specific binding sites and (Na+ + K+)-ATPase inhibition in microsomes from rat heart. Biochem Pharmacol. 1984; 33(1):47-53. DOI: 10.1016/0006-2952(84)90369-1. View

14.

Herzig S, Mohr K . Action of ouabain on rat heart: comparison with its effect on guinea-pig heart. Br J Pharmacol. 1984; 82(1):135-42. PMC: 1987235. DOI: 10.1111/j.1476-5381.1984.tb16450.x. View

15.

Finet M, GODFRAIND T, Noel F . The inotropic effect of ouabain and its antagonism by dihydroouabain in rat isolated atria and ventricles in relation to specific binding sites. Br J Pharmacol. 1983; 80(4):751-9. PMC: 2045062. DOI: 10.1111/j.1476-5381.1983.tb10067.x. View