Poorly Differentiated Clusters Predict a Poor Prognosis for External Auditory Canal Carcinoma

Overview

Journal Head Neck Pathol

Specialties Oncology
Pathology

Date 2018 May 31

PMID 29846906

Citations 7

Authors

Masaru Miyazaki

Mikiko Aoki

Yasuko Okado

Kaori Koga

Makoto Hamasaki

Fumiaki Kiyomi

Toshifumi Sakata

Takashi Nakagawa

Kazuki Nabeshima

Affiliations

Soon will be listed here.

Abstract

Squamous cell carcinoma (SCC) of the external auditory canal (EAC) is rare and offers a poor prognosis; more accurate prognostic biomarkers are required. Our laboratory recently demonstrated that tumor budding, characterized by tumor cell clusters (< 5 cells), and laminin 5-γ2 staining of SCC of the EAC are associated with shorter survival. However, clusters composed of ≥ 5 tumor cells are also found in the stroma. Previous reports of colorectal cancer suggest that poorly differentiated clusters (PDCs) are a negative prognostic indicator. Here, we report on the association between PDCs and prognosis in SCC of the EAC. PDCs and tumor budding were histopathologically and immunohistochemically (cytokeratin AE1/AE3) analyzed in 31 cases of pre-treatment biopsy SCC of the EAC. Clusters in the stroma composed of < or ≥ 5 cancer cells were defined as tumor budding or PDCs, respectively. Entire tumors were initially scanned to identify greatest PDC density. Tumors with low or high PDC density were classified as low- and high-grade, respectively. Patients with high-grade PDCs had a significantly poorer outcome than those with low-grade. Even in cases of low-grade tumor budding, those with high-grade PDCs had a poor prognosis. Multivariate analysis results indicated that high-grade PDCs were associated with poor prognosis. PDC grade can provide a more accurate prognosis than tumor budding in SCC of the EAC.

Citing Articles

Optimizing Prognostic Assessment in High-Risk Head and Neck Squamous Cell Carcinomas: The Impact of Tumor Budding and a Novel Histomorphological Scoring System.

Knebel M, Kuhn J, Korner S, Braun F, Brust L, Wemmert S Cancer Med. 2025; 14(4):e70685.

PMID: 39953822 PMC: 11829162. DOI: 10.1002/cam4.70685.

Grades of Poorly Differentiated Clusters are Associated with Lymph Node and the Tumour, Node and Metastasis Stages in Colorectal Carcinoma.

Kerisnon Krishnan T, Mohtarrudin N, Wan Yaacob W, Hussin H Malays J Med Sci. 2024; 30(6):70-78.

PMID: 38239248 PMC: 10793141. DOI: 10.21315/mjms2023.30.6.8.

Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2.

Komune N, Sato K, Ono M, Imaizumi A, Masuda S, Itoyama S Sci Rep. 2023; 13(1):19636.

PMID: 37949965 PMC: 10638439. DOI: 10.1038/s41598-023-46926-y.

Impact of Positive-Margin Resection of External Auditory Canal Squamous Cell Carcinoma.

Komune N, Kuga R, Hongo T, Kuga D, Sato K, Nakagawa T Cancers (Basel). 2023; 15(17).

PMID: 37686564 PMC: 10486369. DOI: 10.3390/cancers15174289.

Temporal Bone Squamous Cell Carcinoma: Molecular Markers Involved in Carcinogenesis, Behavior, and Prognosis: A Systematic Review.

Alessandrini L, Astolfi L, Franz L, Gentilin E, Mazzoni A, Zanoletti E Int J Mol Sci. 2022; 23(9).

PMID: 35562926 PMC: 9100168. DOI: 10.3390/ijms23094536.

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