Isolation of a Versatile Serratia Marcescens Mutant As a Host and Molecular Cloning of the Aspartase Gene

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Jan 1

PMID 2981795

Citations 16

Authors

T Takagi

M KISUMI

Affiliations

Soon will be listed here.

Abstract

An extracellular nuclease-deficient, antibiotic-sensitive, and restrictionless mutant was isolated from the wild-type strain of Serratia marcescens Sr41 by four rounds of mutagenesis. The mutant was transformed efficiently with plasmid DNAs prepared from Escherichia coli and S. marcescens, and was used as a host for the cloning of the aspartase gene (aspA+) of S. marcescens. Cells carrying the cloned aspA+ gene on a multicopy plasmid produced ca. 39-fold more aspartase than did control cells, and the level of enzyme overproduction was in proportion to the copy number of the aspA+ recombinant plasmid. Aspartase was identified as a polypeptide of molecular weight 52,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Citing Articles

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Shirshikova T, Sierra-Bakhshi C, Kamaletdinova L, Matrosova L, Khabipova N, Evtugyn V mSphere. 2021; 6(2).

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Construction of a Biotin-Overproducing Strain of Serratia marcescens.

Sakurai N, Imai Y, Masuda M, Komatsubara S, Tosa T Appl Environ Microbiol. 1993; 59(9):2857-63.

PMID: 16349036 PMC: 182377. DOI: 10.1128/aem.59.9.2857-2863.1993.

Characterization of a dam mutant of Serratia marcescens and nucleotide sequence of the dam region.

Ostendorf T, Cherepanov P, de Vries J, Wackernagel W J Bacteriol. 1999; 181(13):3880-5.

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Lipase secretion by bacterial hybrid ATP-binding cassette exporters: molecular recognition of the LipBCD, PrtDEF, and HasDEF exporters.

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Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine.

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