Protection of Mice from Wild-type Sendai Virus Infection by a Trypsin-resistant Mutant, TR-2

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1985 Jan 1

PMID 2981341

Citations 12

Authors

M Tashiro

M Homma

Affiliations

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Abstract

A trypsin-resistant mutant of Sendai virus, TR-2, which could be activated by chymotrypsin but not by trypsin or the protease present in mouse lung, was inoculated intranasally into mice after being activated in vitro. TR-2 hardly brought about clinical illness or lung lesions in mice; the protease present in the lung could not activate the progeny virus, and the infection terminated after one-step replication. Nevertheless, the immunoglobulin A antibody against wild-type Sendai virus was produced in the respiratory tracts as well as the serum immunoglobulin G antibody, and the mice were protected from the challenge of the wild-type Sendai virus. On the basis of these results, TR-2 may provide a new model of live vaccine for paramyxoviruses; its availability as a live vaccine is also discussed.

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