Short Tandem Repeat (STR) Profiles of Commonly Used Human Ocular Surface Cell Lines

Overview

Journal Curr Eye Res

Publisher Informa Healthcare

Specialty Ophthalmology

Date 2018 May 23

PMID 29787296

Citations 13

Authors

Alison M McDermott

Hasna Baidouri

Ashley M Woodward

Wendy R Kam

Yang Liu

Xiaomin Chen

Jillian F Ziemanski

Kerry Vistisen

Linda D Hazlett

Kelly K Nichols

Pablo Argueso

David A Sullivan

Affiliations

Soon will be listed here.

Abstract

Purpose: The purpose of this study is to establish the short tandem repeat (STR) profiles of several human cell lines commonly used in ocular surface research.

Materials And Methods: Independently DNA was extracted from multiple passages of three human corneal epithelial cell lines, two human conjunctival epithelial cell lines and one meibomian gland cell line, from different laboratories actively involved in ocular surface research. The samples were then subjected to STR analysis on a fee-for-service basis in an academic setting and the data compared against that in available databases.

Results: The STR profiles for the human corneal epithelial cells were different among the three cell lines studied and for each line the profiles were identical across the samples provided by three laboratories. Profiles for the human conjunctival epithelial cells were different among the two cell lines studied. Profiles for the meibomian gland cell line were identical across the samples provided by three laboratories. No samples were contaminated by elements of other cell lines such as HeLa.

Conclusions: This comprehensive study provides verification of STR profiles for commonly used human ocular surface cell lines that can now be used as a reference by others in the field to authenticate the cell lines in use in their own laboratories.

Citing Articles

Dynasore modulates store-operated calcium entry and mitochondrial calcium release in corneal epithelial cells.

Martinez-Carrasco R, Fini M Exp Eye Res. 2024; 247:110029.

PMID: 39127237 PMC: 11413707. DOI: 10.1016/j.exer.2024.110029.

Evaluation of the effects of latanoprost and benzalkonium chloride on the cell viability and nonpolar lipid profile produced by human meibomian gland epithelial cells in culture.

Ziemanski J, Wilson L, Barnes S, Nichols K Mol Vis. 2024; 29:289-305.

PMID: 38264609 PMC: 10805331.

Aberrations in Cell Signaling Quantified in Diabetic Murine Globes after Injury.

Azzari N, Segars K, Rapaka S, Kushimi L, Rich C, Trinkaus-Randall V Cells. 2024; 13(1).

PMID: 38201230 PMC: 10778404. DOI: 10.3390/cells13010026.

Effect of Carcinomas on Autosomal Trait Screening: A Review Article.

Alhatim H, Abdullah M, Abu Bakar S, Amer S Curr Issues Mol Biol. 2023; 45(9):7275-7285.

PMID: 37754244 PMC: 10529457. DOI: 10.3390/cimb45090460.

Dynasore Protects Corneal Epithelial Cells Subjected to Hyperosmolar Stress in an In Vitro Model of Dry Eye Epitheliopathy.

Martinez-Carrasco R, Fini M Int J Mol Sci. 2023; 24(5).

PMID: 36902183 PMC: 10003680. DOI: 10.3390/ijms24054754.

References

Kaur G, Dufour J . Cell lines: Valuable tools or useless artifacts. Spermatogenesis. 2012; 2(1):1-5. PMC: 3341241. DOI: 10.4161/spmg.19885. View

da Silva E, Coelho Goiato M, da Rocha Bonatto L, de Medeiros R, Dos Santos D, Rangel E . Toxicity analysis of ocular prosthesis acrylic resin with or without pigment incorporation in human conjunctival cell line. Toxicol In Vitro. 2016; 36:180-185. DOI: 10.1016/j.tiv.2016.08.005. View

Gipson I, Spurr-Michaud S, Argueso P, Tisdale A, Ng T, Russo C . Mucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines. Invest Ophthalmol Vis Sci. 2003; 44(6):2496-506. DOI: 10.1167/iovs.02-0851. View

CHANG R . Continuous subcultivation of epithelial-like cells from normal human tissues. Proc Soc Exp Biol Med. 1954; 87(2):440-3. DOI: 10.3181/00379727-87-21406. View

Capes-Davis A, Reid Y, Kline M, Storts D, Strauss E, Dirks W . Match criteria for human cell line authentication: where do we draw the line?. Int J Cancer. 2012; 132(11):2510-9. DOI: 10.1002/ijc.27931. View

Araki-Sasaki K, Ohashi Y, Sasabe T, Hayashi K, Watanabe H, Tano Y . An SV40-immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Vis Sci. 1995; 36(3):614-21. View

. Cell line misidentification: the beginning of the end. Nat Rev Cancer. 2010; 10(6):441-8. DOI: 10.1038/nrc2852. View

Diebold Y, Calonge M, Enriquez de Salamanca A, Callejo S, Corrales R, Saez V . Characterization of a spontaneously immortalized cell line (IOBA-NHC) from normal human conjunctiva. Invest Ophthalmol Vis Sci. 2003; 44(10):4263-74. DOI: 10.1167/iovs.03-0560. View

Rheinwald J, Hahn W, Ramsey M, Wu J, Guo Z, Tsao H . A two-stage, p16(INK4A)- and p53-dependent keratinocyte senescence mechanism that limits replicative potential independent of telomere status. Mol Cell Biol. 2002; 22(14):5157-72. PMC: 139780. DOI: 10.1128/MCB.22.14.5157-5172.2002. View

10.

Folberg R, Kadkol S, Frenkel S, Valyi-Nagy K, Jager M, Peer J . Authenticating cell lines in ophthalmic research laboratories. Invest Ophthalmol Vis Sci. 2008; 49(11):4697-701. PMC: 2576485. DOI: 10.1167/iovs.08-2324. View

11.

Robertson D, Li L, Fisher S, Pearce V, Shay J, Wright W . Characterization of growth and differentiation in a telomerase-immortalized human corneal epithelial cell line. Invest Ophthalmol Vis Sci. 2005; 46(2):470-8. DOI: 10.1167/iovs.04-0528. View

12.

Horbach S, Halffman W . The ghosts of HeLa: How cell line misidentification contaminates the scientific literature. PLoS One. 2017; 12(10):e0186281. PMC: 5638414. DOI: 10.1371/journal.pone.0186281. View

13.

Marx V . Cell-line authentication demystified. Nat Methods. 2014; 11(5):483-8. DOI: 10.1038/nmeth.2932. View

14.

Krishnamoorthy R, Clark A, Daudt D, Vishwanatha J, Yorio T . A forensic path to RGC-5 cell line identification: lessons learned. Invest Ophthalmol Vis Sci. 2013; 54(8):5712-9. DOI: 10.1167/iovs.13-12085. View

15.

Lavappa K . Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro. 1978; 14(5):469-75. DOI: 10.1007/BF02616110. View

16.

Huang Y, Liu Y, Zheng C, Shen C . Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines. PLoS One. 2017; 12(1):e0170384. PMC: 5249119. DOI: 10.1371/journal.pone.0170384. View

17.

Yu X, Ambrosini G, Roszik J, Eterovic A, Stempke-Hale K, Seftor E . Genetic analysis of the 'uveal melanoma' C918 cell line reveals atypical BRAF and common KRAS mutations and single tandem repeat profile identical to the cutaneous melanoma C8161 cell line. Pigment Cell Melanoma Res. 2014; 28(3):357-9. DOI: 10.1111/pcmr.12345. View

18.

Liu S, Hatton M, Khandelwal P, Sullivan D . Culture, immortalization, and characterization of human meibomian gland epithelial cells. Invest Ophthalmol Vis Sci. 2010; 51(8):3993-4005. PMC: 2910637. DOI: 10.1167/iovs.09-5108. View

19.

Akin D, Newman J, McIntyre L, Sugrue S . RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells. Mol Vis. 2016; 22:40-60. PMC: 4734150. View

20.

Wong S, KILBOURNE E . Changing viral susceptibility of a human cell line in continuous cultivation. I. Production of infective virus in a variant of the Chang conjunctival cell following infection with swine or N-WS influenza viruses. J Exp Med. 1961; 113:95-110. PMC: 2137333. DOI: 10.1084/jem.113.1.95. View