» Articles » PMID: 29780253

A 5-serum MiRNA Panel for the Early Detection of Colorectal Cancer

Overview
Publisher Dove Medical Press
Specialty Oncology
Date 2018 May 22
PMID 29780253
Citations 16
Authors
Affiliations
Soon will be listed here.
Abstract

Objective: The study aimed to screen microRNAs (miRNAs) that can be used for the early detection of colorectal cancer (CRC) based on differential expression of miRNA in serum.

Materials And Methods: A three-stage study was designed with a total of 217 CRCs, 168 colorectal adenomas (CRAs), and 190 healthy controls (HCs). A quantitative reverse transcription polymerase chain reaction was performed in three stages. We screened 528 miRNA expression profiles in the sera of 40 patients (CRC n=20, CRA n=10, and HC n=10) for candidate miRNAs, then 210 serum samples (CRC n=90, CRA n=60, and HC n=60) were used for screening of candidate miRNAs. Three hundred and twenty-five independent individual samples (CRC n=107, CRA n=98, and HC n=120) were used to validate the most differentially-expressed miRNAs in the screening stage, and binary logistic regression was used in the validation stage. A receiver operating characteristic curve was drawn to evaluate the diagnostic accuracy.

Results: A 5-serum miRNA panel (miRNA-1246, miRNA-202-3p, miRNA-21-3p, miRNA-1229-3p, and miRNA-532-3p) effectively distinguished CRCs from HCs with 91.6% sensitivity and 91.7% specificity. The area under the curve (AUC) was 0.960 (95% confidence interval [CI]: 0.937-0.983). In addition, the panel also accurately distinguished CRCs from CRAs with 94.4% sensitivity and 84.7% specificity. The AUC was 0.951 (95% CI: 0.922-0.980).

Conclusion: Our 5-serum miRNA panel accurately distinguished CRCs from CRAs and HCs with high sensitivity and specificity. The 5-serum miRNA panel may be a promising prospect for application as a nonintrusive and inexpensive method for the early detection of CRC.

Citing Articles

Linking microRNA to metabolic reprogramming and gut microbiota in the pathogenesis of colorectal cancer (Review).

Bin Wan Mohd Nor W, Kwong S, Fuzi A, Said N, Jamil A, Lee Y Int J Mol Med. 2025; 55(3).

PMID: 39820715 PMC: 11759585. DOI: 10.3892/ijmm.2025.5487.


Revisiting the diagnostic performance of exosomes: harnessing the feasibility of combinatorial exosomal miRNA profiles for colorectal cancer diagnosis.

Park J, Choi J, Hyun D, Byeon C, Kwak S, Park J Discov Oncol. 2024; 15(1):605.

PMID: 39476213 PMC: 11525371. DOI: 10.1007/s12672-024-01481-4.


Advances in microRNAs as Emerging Biomarkers for Colorectal Cancer Early Detection and Diagnosis.

Zdralevic M, Radovic A, Raonic J, Popovic N, Klisic A, Vuckovic L Int J Mol Sci. 2024; 25(20).

PMID: 39456841 PMC: 11507567. DOI: 10.3390/ijms252011060.


Comparison of the diagnostic value of various microRNAs in blood for colorectal cancer: a systematic review and network meta-analysis.

Xu J, Pan L, Wu D, Yao L, Jiang W, Min J BMC Cancer. 2024; 24(1):770.

PMID: 38926893 PMC: 11209970. DOI: 10.1186/s12885-024-12528-8.


Detecting colorectal cancer using genetic and epigenetic biomarkers: screening and diagnosis.

Rezkitha Y, Panenggak N, Lusida M, Rianda R, Mahmudah I, Pradana A J Med Life. 2024; 17(1):4-14.

PMID: 38737656 PMC: 11080499. DOI: 10.25122/jml-2023-0269.


References
1.
Kanaan Z, Roberts H, Eichenberger M, Billeter A, Ocheretner G, Pan J . A plasma microRNA panel for detection of colorectal adenomas: a step toward more precise screening for colorectal cancer. Ann Surg. 2013; 258(3):400-8. DOI: 10.1097/SLA.0b013e3182a15bcc. View

2.
Ohyashiki J, Umezu T, Kobayashi C, Hamamura R, Tanaka M, Kuroda M . Impact on cell to plasma ratio of miR-92a in patients with acute leukemia: in vivo assessment of cell to plasma ratio of miR-92a. BMC Res Notes. 2010; 3:347. PMC: 3022817. DOI: 10.1186/1756-0500-3-347. View

3.
Rice J, Roberts H, Rai S, Galandiuk S . Housekeeping genes for studies of plasma microRNA: A need for more precise standardization. Surgery. 2015; 158(5):1345-51. DOI: 10.1016/j.surg.2015.04.025. View

4.
Gopalakrishnan C, Kamaraj B, Purohit R . Mutations in microRNA binding sites of CEP genes involved in cancer. Cell Biochem Biophys. 2014; 70(3):1933-42. DOI: 10.1007/s12013-014-0153-8. View

5.
Chen X, Liang H, Guan D, Wang C, Hu X, Cui L . A combination of Let-7d, Let-7g and Let-7i serves as a stable reference for normalization of serum microRNAs. PLoS One. 2013; 8(11):e79652. PMC: 3818225. DOI: 10.1371/journal.pone.0079652. View