Comparison of GeneXpert MTB/RIF Assay and LED-FM Microscopy for the Diagnosis of Extra Pulmonary Tuberculosis in Khyber Pakhtunkhwa, Pakistan

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2018 May 13

PMID 29752010

Citations 12

Authors

Anwar Sheed Khan

Sajid Ali

Muhammad Tahir Khan

Sajjad Ahmed

Yasir Khattak

Abduljabbar

Muhammad Irfan

Wasim Sajjad

Affiliations

Soon will be listed here.

Abstract

GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n=390) to 47% (n=347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40-50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.

Citing Articles

[Evaluation of molecular diagnosis of tuberculosis and resistance to rifampicin with GeneXpert MTB/RIF in Algeria].

Yamouni F, Henniche F, Ifticene M, Chabani M, Bensersa D, Ouadah N Med Trop Sante Int. 2024; 4(2).

PMID: 39099717 PMC: 11292432. DOI: 10.48327/mtsi.v4i2.2024.301.

Concordance and discordance of GeneXpert MTB/RIF and conventional culture method for diagnosis of Extra-Pulmonary Tuberculosis at a tertiary care hospital in Pakistan.

Zahid Q, Khursheed N, Adnan F, Zafar A Pak J Med Sci. 2024; 40(2ICON Suppl):S64-S69.

PMID: 38328643 PMC: 10844911. DOI: 10.12669/pjms.40.2(ICON).8967.

Efficacy of Xpert in tuberculosis diagnosis based on various specimens: a systematic review and meta-analysis.

Gong X, He Y, Zhou K, Hua Y, Li Y Front Cell Infect Microbiol. 2023; 13:1149741.

PMID: 37201118 PMC: 10185844. DOI: 10.3389/fcimb.2023.1149741.

The Value of GeneXpert MTB/RIF for Detection in Tuberculosis: A Bibliometrics-Based Analysis and Review.

Li Z J Anal Methods Chem. 2022; 2022:2915018.

PMID: 36284547 PMC: 9588380. DOI: 10.1155/2022/2915018.

Xpert MTB/RIF Ultra versus Xpert MTB/RIF for diagnosis of tuberculous pleural effusion: A systematic review and comparative meta-analysis.

Aggarwal A, Agarwal R, Dhooria S, Prasad K, Sehgal I, Muthu V PLoS One. 2022; 17(7):e0268483.

PMID: 35816471 PMC: 9273090. DOI: 10.1371/journal.pone.0268483.

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