NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2018 May 11

PMID 29743812

Citations 7

Authors

Abdullah Basoglu

Ismail Sen

Gaia Meoni

Leonardo Tenori

Amir Naseri

Affiliations

Soon will be listed here.

Abstract

The aim of this first study was to reveal the new potential biomarkers by a metabolomics approach in severe septic calves. Sepsis is a common cause of morbidity and mortality in newborn dairy calves. The main challenges with the use of biomarkers of sepsis in domestic animals are their availability, cost, and time required to obtain a result. Metabolomics may offer the potential to identify biomarkers that define calf sepsis in terms of combined clinical, physiological, and pathobiological abnormalities. To our knowledge, this is the first study presenting an NMR- (nuclear magnetic resonance-) based plasma metabolomics at set intervals in neonatal septic calves. Twenty neonatal dairy calves with severe sepsis and ten healthy calves were used. Hematological and biochemical health profiles were gathered in plasma samples at set intervals. Similarly, NMR spectra were acquired. All diseased animals (except one) died after 72 hours. Clinical and laboratory results were in accordance with those of severe septic animals. Multivariate analysis on NMR plasma spectra proved to be an excellent tool for faster identification of calves with severe sepsis from healthy animals. The NMR-based metabolomic profile may contribute to the better understanding of severe sepsis in newborn calves.

Citing Articles

Optimization of Antimicrobial Use for Sepsis in Calves: Bayesian Evaluation of Existing and Novel Sepsis Scores.

Pas M, Bokma J, Boyen F, Chantillon L, Castelain D, Clinquart J Animals (Basel). 2025; 15(4).

PMID: 40003069 PMC: 11851423. DOI: 10.3390/ani15040586.

Neopterin, procalcitonin, clinical biochemistry, and hematology in calves with neonatal sepsis.

Akyuz E, Gokce G Trop Anim Health Prod. 2021; 53(3):354.

PMID: 34106342 PMC: 8187137. DOI: 10.1007/s11250-021-02779-z.

Metabolic Fingerprinting of Feces from Calves, Subjected to Gram-Negative Bacterial Endotoxin.

Kamel Oroumieh S, Naserian A, Van Meulebroek L, De Paepe E, Valizadeh R, Vanhaecke L Metabolites. 2021; 11(2).

PMID: 33668448 PMC: 7918135. DOI: 10.3390/metabo11020108.

Metabolomics in the study of spontaneous animal diseases.

Tran H, McConville M, Loukopoulos P J Vet Diagn Invest. 2020; 32(5):635-647.

PMID: 32807042 PMC: 7488963. DOI: 10.1177/1040638720948505.

Serum Metabolomics Revealed the Differential Metabolic Pathway in Calves with Severe Clinical Diarrhea Symptoms.

Huang M, Cui D, Wu X, Hui W, Yan Z, Ding X Animals (Basel). 2020; 10(5).

PMID: 32354125 PMC: 7278412. DOI: 10.3390/ani10050769.

References

Xia J, Sinelnikov I, Han B, Wishart D . MetaboAnalyst 3.0--making metabolomics more meaningful. Nucleic Acids Res. 2015; 43(W1):W251-7. PMC: 4489235. DOI: 10.1093/nar/gkv380. View

Noto A, Fanos V, Dessi A . Metabolomics in Newborns. Adv Clin Chem. 2016; 74:35-61. DOI: 10.1016/bs.acc.2015.12.006. View

Santos C . Shedding metabo'light' on the search for sepsis biomarkers. Crit Care. 2015; 19:277. PMC: 4493818. DOI: 10.1186/s13054-015-0969-7. View

Beckonert O, Keun H, Ebbels T, Bundy J, Holmes E, Lindon J . Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts. Nat Protoc. 2007; 2(11):2692-703. DOI: 10.1038/nprot.2007.376. View

Gowda G, Raftery D . Quantitating metabolites in protein precipitated serum using NMR spectroscopy. Anal Chem. 2014; 86(11):5433-40. PMC: 4045325. DOI: 10.1021/ac5005103. View

Liu Z, Yin P, Amathieu R, Savarin P, Xu G . Application of LC-MS-based metabolomics method in differentiating septic survivors from non-survivors. Anal Bioanal Chem. 2016; 408(27):7641-7649. DOI: 10.1007/s00216-016-9845-9. View

Bunger R, Mallet R . Metabolomics and Receiver Operating Characteristic Analysis: A Promising Approach for Sepsis Diagnosis. Crit Care Med. 2016; 44(9):1784-5. PMC: 4988321. DOI: 10.1097/CCM.0000000000001795. View

Prucha M, Bellingan G, Zazula R . Sepsis biomarkers. Clin Chim Acta. 2014; 440:97-103. DOI: 10.1016/j.cca.2014.11.012. View

Englert J, Rogers A . Metabolism, Metabolomics, and Nutritional Support of Patients with Sepsis. Clin Chest Med. 2016; 37(2):321-31. PMC: 5084839. DOI: 10.1016/j.ccm.2016.01.011. View

10.

Gilfillan M, Bhandari V . Biomarkers for the diagnosis of neonatal sepsis and necrotizing enterocolitis: Clinical practice guidelines. Early Hum Dev. 2017; 105:25-33. DOI: 10.1016/j.earlhumdev.2016.12.002. View

11.

Levy M, Fink M, Marshall J, Abraham E, Angus D, Cook D . 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Intensive Care Med. 2003; 29(4):530-8. DOI: 10.1007/s00134-003-1662-x. View

12.

Fanos V, Caboni P, Corsello G, Stronati M, Gazzolo D, Noto A . Urinary (1)H-NMR and GC-MS metabolomics predicts early and late onset neonatal sepsis. Early Hum Dev. 2014; 90 Suppl 1:S78-83. DOI: 10.1016/S0378-3782(14)70024-6. View

13.

Mussap M, Antonucci R, Noto A, Fanos V . The role of metabolomics in neonatal and pediatric laboratory medicine. Clin Chim Acta. 2013; 426:127-38. DOI: 10.1016/j.cca.2013.08.020. View

14.

Dessi A, Corsello G, Stronati M, Gazzolo D, Caboni P, Carboni R . New diagnostic possibilities in systemic neonatal infections: metabolomics. Early Hum Dev. 2014; 90 Suppl 1:S19-21. DOI: 10.1016/S0378-3782(14)70007-6. View

15.

Kauppi A, Edin A, Ziegler I, Molling P, Sjostedt A, Gylfe A . Metabolites in Blood for Prediction of Bacteremic Sepsis in the Emergency Room. PLoS One. 2016; 11(1):e0147670. PMC: 4723089. DOI: 10.1371/journal.pone.0147670. View

16.

Dona A, Jimenez B, Schafer H, Humpfer E, Spraul M, Lewis M . Precision high-throughput proton NMR spectroscopy of human urine, serum, and plasma for large-scale metabolic phenotyping. Anal Chem. 2014; 86(19):9887-94. DOI: 10.1021/ac5025039. View

17.

Noto A, Mussap M, Fanos V . Is 1H NMR metabolomics becoming the promising early biomarker for neonatal sepsis and for monitoring the antibiotic toxicity?. J Chemother. 2013; 26(3):130-2. DOI: 10.1179/1973947813Y.0000000149. View

18.

Garcia-Simon M, Morales J, Modesto-Alapont V, Gonzalez-Marrachelli V, Vento-Rehues R, Jorda-Minana A . Prognosis Biomarkers of Severe Sepsis and Septic Shock by 1H NMR Urine Metabolomics in the Intensive Care Unit. PLoS One. 2015; 10(11):e0140993. PMC: 4643898. DOI: 10.1371/journal.pone.0140993. View

19.

Langley R, Wong H . Early Diagnosis of Sepsis: Is an Integrated Omics Approach the Way Forward?. Mol Diagn Ther. 2017; 21(5):525-537. PMC: 6147261. DOI: 10.1007/s40291-017-0282-z. View

20.

Wu G . Amino acids: metabolism, functions, and nutrition. Amino Acids. 2009; 37(1):1-17. DOI: 10.1007/s00726-009-0269-0. View