Serologic Tests of IgG and IgM Antibodies and IgG Avidity for Diagnosis of Ocular Toxoplasmosis

Overview

Journal Korean J Parasitol

Specialty Parasitology

Date 2018 May 11

PMID 29742869

Citations 7

Authors

Bahman Rahimi-Esboei

Mohammad Zarei

Mehdi Mohebali

Hossein Keshavarz Valian

Saeedeh Shojaee

Raziyeh Mahmoudzadeh

Mirataollah Salabati

Affiliations

Soon will be listed here.

Abstract

This prospective study was aimed to detect acute and chronic ocular toxoplasmosis by comparison of anti- IgM and IgG antibody levels and IgG avidity test. One hundred and seventeen patients with ocular toxoplasmosis (OT) who referred to the Farabi Eye Hospital, Tehran, Iran were included in this study. Of the patients, 77 cases were positive for anti- IgG, and 8 cases were positive for anti- IgM. IgG avidity test revealed 11, 4, and 102 cases were low, intermediate, and high, respectively, and 6.8% and 9.4% of cases were positive for IgM and IgG avidity tests, respectively (=0.632). Agreement (Kappa value) between paired tests IgG-IgM, IgG-IgG avidity, and IgM-IgG avidity was 0.080, 0.099, and 0.721, respectively (<0.05). This study showed that conventional serologic tests (IgM and IgG levels) and IgG avidity correlate well each other and can be used to differentiate recent infections from old OT. It seems that reactivated old infections rather than recently acquired infections are majority of Iranian OT patients.

Citing Articles

Efficacy of amniotic fluid, blood and urine samples for the diagnosis of toxoplasmosis in pregnant women candidates for amniocentesis using serological and molecular techniques.

Abedian R, Esboei B, Kordi S, Roshan H, Hezarjaribi H, Rahmani Z BMC Pregnancy Childbirth. 2024; 24(1):771.

PMID: 39578753 PMC: 11583409. DOI: 10.1186/s12884-024-06979-x.

Identification of novel biomarkers for anti- IgM detection and the potential application in rapid diagnostic fluorescent tests.

Nguyen M, Yeo S, Park H Front Microbiol. 2024; 15:1385582.

PMID: 38894968 PMC: 11184589. DOI: 10.3389/fmicb.2024.1385582.

Role of IgG avidity in eyes with active Toxoplasma retinochoroiditis.

Lakhmawar P, Kelgaonkar A, Patel A, Khalsa A, Basu S Indian J Ophthalmol. 2024; 72(Suppl 4):S601-S605.

PMID: 38324620 PMC: 11338399. DOI: 10.4103/IJO.IJO_3000_23.

Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene.

Esboei B, Fallahi S, Zarei M, Kazemi B, Mohebali M, Shojaee S BMC Infect Dis. 2022; 22(1):89.

PMID: 35078413 PMC: 8787932. DOI: 10.1186/s12879-022-07073-3.

Genotyping and phylogenic study of isolates from human keratitis and swimming pool water samples in Iran.

Esboei B, Fakhar M, Saberi R, Barati M, Moslemi M, Hassannia H Parasite Epidemiol Control. 2020; 11:e00164.

PMID: 32671234 PMC: 7341446. DOI: 10.1016/j.parepi.2020.e00164.

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