Osmotic Modulation of Chromatin Impacts on Efficiency and Kinetics of Cell Fate Modulation

Overview

Journal Sci Rep

Specialty Science

Date 2018 May 10

PMID 29740078

Citations 8

Authors

A F Lima

G May

J Diaz-Colunga

S Pedreiro

A Paiva

L Ferreira

T Enver

F J Iborra

R Pires das Neves

Affiliations

Soon will be listed here.

Abstract

Chromatin structure is a major regulator of transcription and gene expression. Herein we explore the use of osmotic modulation to modify the chromatin structure and reprogram gene expression. In this study we use the extracellular osmotic pressure as a chromatin structure and transcriptional modulator. Hyposmotic modulation promotes chromatin loosening and induces changes in RNA polymerase II (Pol II) activity. The chromatin decondensation opens space for higher amounts of DNA engaged RNA Pol II. Hyposmotic modulation constitutes an alternative route to manipulate cell fate decisions. This technology was tested in model protocols of induced pluripotency and transdifferentiation in cells growing in suspension and adherent to substrates, CD34 umbilical-cord-blood (UCB), fibroblasts and B-cells. The efficiency and kinetics of these cell fate modulation processes were improved by transient hyposmotic modulation of the cell environment.

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