The Hypoxia-Mimetic Agent Cobalt Chloride Differently Affects Human Mesenchymal Stem Cells in Their Chondrogenic Potential

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2018 May 8

PMID 29731777

Citations 19

Authors

Gabriella Teti

Stefano Focaroli

Viviana Salvatore

Eleonora Mazzotti

Laura Ingra

Antonio Mazzotti

Mirella Falconi

Affiliations

Soon will be listed here.

Abstract

Adult stem cells are a promising cell source for cartilage regeneration. They resided in a special microenvironment known as the stem-cell niche, characterized by the presence of low oxygen concentration. Cobalt chloride (CoCl) imitates hypoxia in vitro by stabilizing hypoxia-inducible factor-alpha (HIF-1), which is the master regulator in the cellular adaptive response to hypoxia. In this study, the influence of CoCl on the chondrogenic potential of human MSCs, isolated from dental pulp, umbilical cord, and adipose tissue, was investigated. Cells were treated with concentrations of CoCl ranging from 50 to 400 M. Cell viability, HIF-1 protein synthesis, and the expression of the chondrogenic markers were analyzed. The results showed that the CoCl supplementation had no effect on cell viability, while the upregulation of chondrogenic markers such as SOX9, COL2A1, VCAN, and ACAN was dependent on the cellular source. This study shows that hypoxia, induced by CoCl treatment, can differently influence the behavior of MSCs, isolated from different sources, in their chondrogenic potential. These findings should be taken into consideration in the treatment of cartilage repair and regeneration based on stem cell therapies.

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