Priming with Caffeic Acid Enhances the Potential and Survival Ability of Human Adipose-derived Stem Cells to Counteract Hypoxia

Overview

Journal Regen Ther

Date 2023 Feb 8

PMID 36751276

Authors

H M Shifa Ul Haq

Ramla Ashfaq

Azra Mehmood

Warda Shahid

Ghufran Azam

Maryam Azam

Saba Tasneem

Shehla Javed Akram

Kausar Malik

Sheikh Riazuddin

Affiliations

Soon will be listed here.

Abstract

The therapeutic effectiveness of stem cells after transplantation is hampered by the hypoxic milieu of chronic wounds. Prior research has established antioxidant priming as a thorough plan to improve stem cell performance. The purpose of this study was to ascertain how caffeic acid (CA) priming affected the ability of human adipose-derived stem cells (hASCs) to function under hypoxic stress. In order to study the cytoprotective properties of CA, hASCs were primed with CA in CoCl hypoxic conditions. Microscopy was used to assess cell morphology, while XTT, Trypan Blue, X-gal, LDH, Live Dead, scratch wound healing, and ROS assays were used to analyze viability, senescence, cell death, proliferation, and reactive oxygen species prevalence in the cells. According to our findings, CA priming enhances hASCs' ability to survive and regenerate in a hypoxic microenvironment more effectively than untreated hASCs. Our in-vitro research suggested that pre-treatment with CA of hASCs could be a unique way to enhance their therapeutic efficacy and ability to survive in hypoxic microenvironments.

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