Gold Nanoparticle-based Colorimetric Method for the Detection of Prostate-specific Antigen

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2018 May 8

PMID 29731627

Citations 4

Authors

Ning Xia

Dehua Deng

Yiru Wang

Chao Fang

Su-Juan Li

Affiliations

Soon will be listed here.

Abstract

Background: Prostate-specific antigen (PSA), a serine protease, is a biomarker for preoperative diagnosis and screening of prostate cancer and monitoring of its posttreatment.

Methods: In this work, we reported a colorimetric method for clinical detection of PSA using gold nanoparticles (AuNPs) as the reporters. The method is based on ascorbic acid (AA)-induced in situ formation of AuNPs and Cu-catalyzed oxidation of AA. Specifically, HAuCl can be reduced into AuNPs by AA; Cu ion can catalyze the oxidation of AA by O to inhibit the formation of AuNPs. In the presence of the PSA-specific peptide (DASSKLQLAPP)-modified gold-coated magnetic microbeads (MMBs; denoted as DASSKLQLAPP-MMBs), complexation of Cu by the MMBs through the DAH-Cu interaction depressed the catalyzed oxidation of AA and thus allowed for the formation of red AuNPs. However, once the peptide immobilized on the MMB surface was cleaved by PSA, the DASSKLQ segment would be released. The resultant LAPP fragment remaining on the MMB surface could not sequestrate Cu to depress its catalytic activity toward AA oxidation. Consequently, no or less AuNPs were generated.

Results: The linear range for PSA detection was found to be 0~0.8 ng/mL with a detection limit of 0.02 ng/mL. Because of the separation of cleavage step and measurement step, the interference of matrix components in biological samples was avoided.

Conclusion: The high extinction coefficient of AuNPs facilitates the colorimetric analysis of PSA in serum samples. This work is helpful for designing of other protease biosensors by matching specific peptide substrates.

Citing Articles

Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer.

Ivanov Y, Malsagova K, Goldaeva K, Kapustina S, Pleshakova T, Popov V Sensors (Basel). 2023; 23(17).

PMID: 37687982 PMC: 10490786. DOI: 10.3390/s23177527.

Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer.

Appidi T, Mudigunda S, Kodandapani S, Rengan A Nanoscale Adv. 2022; 2(12):5737-5745.

PMID: 36133887 PMC: 9419083. DOI: 10.1039/d0na00686f.

Nanotreatment and Nanodiagnosis of Prostate Cancer: Recent Updates.

Barani M, Sabir F, Rahdar A, Arshad R, Kyzas G Nanomaterials (Basel). 2020; 10(9).

PMID: 32872181 PMC: 7559844. DOI: 10.3390/nano10091696.

Fiber optic sensor based on ZnO nanowires decorated by Au nanoparticles for improved plasmonic biosensor.

Kim H, Park J, Lee S Sci Rep. 2019; 9(1):15605.

PMID: 31666617 PMC: 6821738. DOI: 10.1038/s41598-019-52056-1.

References

Suaifan G, Shehadeh M, Al-Ijel H, Ng A, Zourob M . Recent progress in prostate-specific antigen and HIV proteases detection. Expert Rev Mol Diagn. 2013; 13(7):707-18. DOI: 10.1586/14737159.2013.835576. View

Chen C, Huang C, Chang H . Label-free colorimetric detection of picomolar thrombin in blood plasma using a gold nanoparticle-based assay. Biosens Bioelectron. 2010; 25(8):1922-7. DOI: 10.1016/j.bios.2010.01.005. View

Baron R, Zayats M, Willner I . Dopamine-, L-DOPA-, adrenaline-, and noradrenaline-induced growth of Au nanoparticles: assays for the detection of neurotransmitters and of tyrosinase activity. Anal Chem. 2005; 77(6):1566-71. DOI: 10.1021/ac048691v. View

Suaifan G, Esseghaier C, Ng A, Zourob M . Ultra-rapid colorimetric assay for protease detection using magnetic nanoparticle-based biosensors. Analyst. 2013; 138(13):3735-9. DOI: 10.1039/c3an36881e. View

Ibau C, Md Arshad M, Gopinath S . Current advances and future visions on bioelectronic immunosensing for prostate-specific antigen. Biosens Bioelectron. 2017; 98:267-284. DOI: 10.1016/j.bios.2017.06.049. View

Liu L, Jiang D, McDonald A, Hao Y, Millhauser G, Zhou F . Copper redox cycling in the prion protein depends critically on binding mode. J Am Chem Soc. 2011; 133(31):12229-37. PMC: 3166251. DOI: 10.1021/ja2045259. View

Sun C, Su K, Valentine J, Rosa-Bauza Y, Ellman J, Elboudwarej O . Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors. ACS Nano. 2010; 4(2):978-84. PMC: 2884196. DOI: 10.1021/nn900757p. View

Choi J, Kim H, Choi J, Hong J, Kim Y, Oh B . A novel Au-nanoparticle biosensor for the rapid and simple detection of PSA using a sequence-specific peptide cleavage reaction. Biosens Bioelectron. 2013; 49:415-9. DOI: 10.1016/j.bios.2013.05.042. View

Guarise C, Pasquato L, De Filippis V, Scrimin P . Gold nanoparticles-based protease assay. Proc Natl Acad Sci U S A. 2006; 103(11):3978-82. PMC: 1449631. DOI: 10.1073/pnas.0509372103. View

10.

Xue W, Zhang G, Zhang D . A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles. Analyst. 2011; 136(15):3136-41. DOI: 10.1039/c1an15224f. View

11.

Folk D, Franz K . A prochelator activated by beta-secretase inhibits Abeta aggregation and suppresses copper-induced reactive oxygen species formation. J Am Chem Soc. 2010; 132(14):4994-5. PMC: 2860781. DOI: 10.1021/ja100943r. View

12.

Shiigi H, Kinoshita T, Shibutani N, Nishino T, Nagaoka T . Efficient collection and sensitive detection using conducting magnetic microbeads. Anal Chem. 2014; 86(10):4977-81. DOI: 10.1021/ac500452w. View

13.

Tang Z, Fu Y, Ma Z . Bovine serum albumin as an effective sensitivity enhancer for peptide-based amperometric biosensor for ultrasensitive detection of prostate specific antigen. Biosens Bioelectron. 2017; 94:394-399. DOI: 10.1016/j.bios.2017.03.030. View

14.

Liu D, Yang J, Wang H, Wang Z, Huang X, Wang Z . Glucose oxidase-catalyzed growth of gold nanoparticles enables quantitative detection of attomolar cancer biomarkers. Anal Chem. 2014; 86(12):5800-6. PMC: 4066917. DOI: 10.1021/ac500478g. View

15.

Gao Z, Xu M, Hou L, Chen G, Tang D . Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules. Anal Chem. 2013; 85(14):6945-52. DOI: 10.1021/ac401433p. View

16.

Feng T, Feng D, Shi W, Li X, Ma H . A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen. Mol Biosyst. 2012; 8(5):1441-5. DOI: 10.1039/c2mb05379a. View

17.

Thavanathan J, Huang N, Thong K . Colorimetric biosensing of targeted gene sequence using dual nanoparticle platforms. Int J Nanomedicine. 2015; 10:2711-22. PMC: 4396418. DOI: 10.2147/IJN.S74753. View

18.

Ding X, Yang K . Quantitative serine protease assays based on formation of copper(II)-oligopeptide complexes. Analyst. 2014; 140(1):340-5. DOI: 10.1039/c4an01731e. View

19.

Kim C, Lee D, Kim C, Lee K, Lee C, Ahn I . Gold nanoparticles-based colorimetric assay for cathepsin B activity and the efficiency of its inhibitors. Anal Chem. 2014; 86(8):3825-33. DOI: 10.1021/ac4039064. View

20.

Zhang J, Qi H, Li Z, Zhang N, Gao Q, Zhang C . Electrogenerated Chemiluminescence Bioanalytic System Based on Biocleavage of Probes and Homogeneous Detection. Anal Chem. 2015; 87(13):6510-5. DOI: 10.1021/acs.analchem.5b01396. View