Development of in Situ Hybridization for Detection of Elephant Endotheliotropic Herpesvirus in Asian Elephants

Overview

Journal J Vet Diagn Invest

Specialty Veterinary Medicine

Date 2018 May 8

PMID 29730973

Citations 6

Authors

Varankpicha Kochakul

Kittikorn Boonsri

Saruda Tiwananthagorn

Chalermchart Somgird

Chatchote Thitaram

Kidsadagon Pringproa

Affiliations

Soon will be listed here.

Abstract

Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asian elephants ( Elephas maximus). Digoxigenin (DIG) DNA probes from the polymerase and terminase genes of EEHV were synthesized using a PCR DIG-labeling method, and detection of hybridized probe to target EEHV DNA was carried out by anti-DIG immunolabeling. Distribution of EEHV-1A and EEHV-4 genomes was found to be prominent in mononuclear phagocytic cells of spleen and endothelial cells of visceral organs. ISH enables the detection of EEHV infection and has applications in understanding pathogenesis of EEHV in Asian elephants.

Citing Articles

Development of Nonstructural Protein-Based Indirect ELISA to Identify Elephant Endotheliotropic Herpesvirus (EEHV) Infection in Asian Elephants ().

Guntawang T, Sittisak T, Tankaew P, Thitaram C, Langkapin V, Angkawanish T Animals (Basel). 2022; 12(14).

PMID: 35883294 PMC: 9312089. DOI: 10.3390/ani12141747.

Elephant Endotheliotropic Herpesvirus 1, 4 and 5 in China: Occurrence in Multiple Sample Types and Implications for Wild and Captive Population Surveillance.

Yang N, Bao M, Zhu B, Shen Q, Guo X, Li W Viruses. 2022; 14(2).

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The occurrence of elephant endotheliotropic herpesvirus infection in wild and captive Asian elephants in Thailand: Investigation based on viral DNA and host antibody.

Prompiram P, Wiriyarat W, Bhusri B, Paungpin W, Jairak W, Sripiboon S Vet World. 2021; 14(2):545-550.

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Feline bocavirus-1 associated with outbreaks of hemorrhagic enteritis in household cats: potential first evidence of a pathological role, viral tropism and natural genetic recombination.

Piewbang C, Kasantikul T, Pringproa K, Techangamsuwan S Sci Rep. 2019; 9(1):16367.

PMID: 31705016 PMC: 6841677. DOI: 10.1038/s41598-019-52902-2.

Possible roles of monocytes/macrophages in response to elephant endotheliotropic herpesvirus (EEHV) infections in Asian elephants (Elephas maximus).

Srivorakul S, Guntawang T, Kochagul V, Photichai K, Sittisak T, Janyamethakul T PLoS One. 2019; 14(9):e0222158.

PMID: 31491031 PMC: 6730851. DOI: 10.1371/journal.pone.0222158.

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