Sex Determination in Is Accompanied by Transcriptome Changes That Drive Epigenetic Reprogramming of the Young Gametophyte

Overview

Journal G3 (Bethesda)

Publisher Oxford University Press

Specialties Genetics
Molecular Biology

Date 2018 May 4

PMID 29720393

Citations 10

Authors

Nadia M Atallah

Olga Vitek

Federico Gaiti

Milos Tanurdzic

Jo Ann Banks

Affiliations

Soon will be listed here.

Abstract

The fern is an important model for studies of sex determination and gamete differentiation in homosporous plants. Here we use RNA-seq to assemble a transcriptome and identify genes differentially expressed in young gametophytes as their sex is determined by the presence or absence of the male-inducing pheromone called antheridiogen. Of the 1,163 consensus differentially expressed genes identified, the vast majority (1,030) are up-regulated in gametophytes treated with antheridiogen. GO term enrichment analyses of these DEGs reveals that a large number of genes involved in epigenetic reprogramming of the gametophyte genome are up-regulated by the pheromone. Additional hormone response and development genes are also up-regulated by the pheromone. This gametophyte transcriptome and gene expression dataset will prove useful for studies focusing on sex determination and differentiation in plants.

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