The Effects of Pertussis Toxin on Human T Lymphocytes

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1988 Jul 1

PMID 2970430

Citations 6

Authors

E A Macintyre

P E Tatham

D C Linch

Affiliations

Soon will be listed here.

Abstract

Purified pertussis toxin (PPT) is a potent mitogen for human T lymphocytes and is shown to cause rapid calcium mobilization in resting T cells, a T-cell line and CD3- lymphocytes with natural killer (NK) activity. In resting T cells the PPT activation is associated with cytoplasmic alkalinization. A similar rise in intracellular free calcium ([Ca2+]i) and cytoplasmic alkalinization is observed with activation through the antigen receptor complex. The effect of PPT is unlikely to be mediated through this pathway, however, as it can mobilize calcium in lymphocytes that do not express the CD3-Ti complex. In contrast to several other cell types, re-incubation of resting human T cells with PPT, up to a dose of 100 ng/ml for 2 hr does not block subsequent agonist-induced calcium mobilization dependent on G protein-mediated phospholipase C activation. Mitogenic doses of PPT cause a modest reduction in subsequent agonist responses, but this is likely to be due to a post-activation refractory state rather than G protein inactivation.

Citing Articles

Pertussis toxin activates platelets through an interaction with platelet glycoprotein Ib.

Sindt K, Hewlett E, Redpath G, Rappuoli R, Gray L, Vandenberg S Infect Immun. 1994; 62(8):3108-14.

PMID: 8039878 PMC: 302934. DOI: 10.1128/iai.62.8.3108-3114.1994.

Binding of monoclonal antibody to CD16 causes calcium mobilization in large granular lymphocytes but inhibits NK killing.

Macintyre E, Wallace D, OFlynn K, Tetteroo P, MORGAN G, Linch D Immunology. 1989; 66(3):459-65.

PMID: 2564843 PMC: 1385237.

The receptors for ATP and fMetLeuPhe are independently coupled to phospholipases C and A2 via G-protein(s). Relationship between phospholipase C and A2 activation and exocytosis in HL60 cells and human neutrophils.

Cockcroft S, Stutchfield J Biochem J. 1989; 263(3):715-23.

PMID: 2512911 PMC: 1133491. DOI: 10.1042/bj2630715.

Interactive effects of pertussis toxin and the phorbol ester tumour promotor, phorbol dibutyrate, on T-lymphocyte mitogenesis and the expression of phenotypic determinants.

Strnad C, Lin W, Carchman R Immunology. 1989; 66(4):539-45.

PMID: 2469642 PMC: 1385154.

Lymphocyte receptors for pertussis toxin.

Clark C, Armstrong G Infect Immun. 1990; 58(12):3840-6.

PMID: 2123822 PMC: 313744. DOI: 10.1128/iai.58.12.3840-3846.1990.

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