RNA Binding Protein, HuR, Regulates Expression Through Stabilizing MEF2C Transcription Factor MRNA

Overview

Journal J Am Heart Assoc

Specialty Cardiology & Vascular Diseases

Date 2018 Apr 22

PMID 29678826

Citations 14

Authors

Anyu Zhou

Guangbin Shi

Gyeoung-Jin Kang

An Xie

Hong Liu

Ning Jiang

Man Liu

Euy-Myoung Jeong

Samuel C Dudley Jr

Affiliations

Soon will be listed here.

Abstract

Background: Although transcription is the initial process of gene expression, posttranscriptional gene expression regulation has also played a critical role for fine-tuning gene expression in a fast, precise, and cost-effective manner. Although the regulation of sodium channel α-subunit () mRNA expression has been studied at both transcriptional and pre-mRNA splicing levels, the molecular mechanisms governing mRNA expression are far from clear.

Methods And Results: Herein, we show that, as evidenced by ribonucleoprotein immunoprecipitation assay, RNA binding protein Hu antigen R/ELAV like RNA binding protein 1 (HuR/ELAVL1) and myocyte enhancer factor-2C (MEF2C) transcription factor mRNA are associated. HuR positively regulated transcription factor MEF2C mRNA expression by protecting its mRNA from degradation. As demonstrated by both chromatin immunoprecipitation-quantitative polymerase chain reaction assay and an electrophoretic mobility shift assay, MEF2C enhanced transcription by binding to a putative MEF2C binding site within promoter region. Overexpression of HuR increased the expression of mRNA, and this effect was attenuated by the presence of MEF2C small interfering RNA in cardiomyocytes.

Conclusions: In conclusion, our results suggested that HuR participates in a combined network at the DNA and RNA levels that regulates mRNA expression. HuR upregulates MEF2C mRNA expression by protecting MEF2C mRNA from degradation, and consequently, the elevated MEF2C enhances mRNA transcription.

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