A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells Associated with Enhanced Wound Healing

Overview

Journal Sci Rep

Specialty Science

Date 2018 Apr 20

PMID 29670130

Citations 15

Authors

Abhijit Basu

Saira Munir

Medanie A Mulaw

Karmveer Singh

Diana Crisan

Anca Sindrilaru

Nicolai Treiber

Meinhard Wlaschek

Markus Huber-Lang

Florian Gebhard

Karin Scharffetter-Kochanek

Affiliations

Soon will be listed here.

Abstract

We here investigated whether the unique capacity of mesenchymal stem cells (MSCs) to re-establish tissue homeostasis depends on their potential to sense danger associated molecular pattern (DAMP) and to mount an adaptive response in the interest of tissue repair. Unexpectedly, after injection of MSCs which had been pretreated with the calcium-binding DAMP protein S100A8/A9 into murine full-thickness wounds, we observed a significant acceleration of healing even exceeding that of non-treated MSCs. This correlates with a fundamental reprogramming of the transcriptome in S100A8/A9 treated MSCs as deduced from RNA-seq analysis and its validation. A network of genes involved in proteolysis, macrophage phagocytosis, and inflammation control profoundly contribute to the clean-up of the wound site. In parallel, miR582-5p and genes boosting energy and encoding specific extracellular matrix proteins are reminiscent of scar-reduced tissue repair. This unprecedented finding holds substantial promise to refine current MSC-based therapies for difficult-to-treat wounds and fibrotic conditions.

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