In Vitro Fertilization in Mice

Overview

Journal Cold Spring Harb Protoc

Specialties Biomedical Engineering
Pathology

Date 2018 Apr 20

PMID 29669849

Citations 11

Authors

Toru Takeo

Naomi Nakagata

Affiliations

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Abstract

The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).

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