Identification of Entomopathogenic Nematodes and Symbiotic Bacteria from Nam Nao National Park in Thailand and Larvicidal Activity of Symbiotic Bacteria Against Aedes Aegypti and Aedes Albopictus

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 Apr 12

PMID 29641570

Citations 18

Authors

Temsiri Yooyangket

Paramaporn Muangpat

Raxsina Polseela

Sarunporn Tandhavanant

Aunchalee Thanwisai

Apichat Vitta

Affiliations

Soon will be listed here.

Abstract

Entomopathogenic nematodes (EPNs) that are symbiotically associated with Xenorhabdus and Photorhabdus bacteria can kill target insects via direct infection and toxin action. There are limited reports identifying such organisms in the National Park of Thailand. Therefore, the objectives of this study were to identify EPNs and symbiotic bacteria from Nam Nao National Park, Phetchabun Province, Thailand and to evaluate the larvicidal activity of bacteria against Aedes aegypti and Ae. albopictus. A total of 12 EPN isolates belonging to Steinernema and Heterorhabditis were obtained form 940 soil samples between February 2014 and July 2016. EPNs were molecularly identified as S. websteri (10 isolates) and H. baujardi (2 isolates). Symbiotic bacteria were isolated from EPNs and molecularly identified as P. luminescens subsp. akhurstii (13 isolates), X. stockiae (11 isolates), X. vietnamensis (2 isolates) and X. japonica (1 isolate). For the bioassay, bacterial suspensions were evaluated for toxicity against third to early fourth instar larvae of Aedes spp. The larvae of both Aedes species were orally susceptible to symbiotic bacteria. The highest larval mortality of Ae. aegypti was 99% after exposure to X. stockiae (bNN112.3_TH) at 96 h, and the highest mortality of Ae. albopictus was 98% after exposure to P. luminescens subsp. akhurstii (bNN121.4_TH) at 96 h. In contrast to the control groups (Escherichia coli and distilled water), the mortality rate of both mosquito larvae ranged between 0 and 7% at 72 h. Here, we report the first observation of X. vietnamensis in Thailand. Additionally, we report the first observation of P. luminescens subsp. akhurstii associated with H. baujardi in Thailand. X. stockiae has potential to be a biocontrol agent for mosquitoes. This investigation provides a survey of the basic diversity of EPNs and symbiotic bacteria in the National Park of Thailand, and it is a bacterial resource for further studies of bioactive compounds.

Citing Articles

Symbiotic bacteria associated with entomopathogenic nematodes showed molluscicidal activity against Biomphalaria glabrata, an intermediate host of Schistosoma mansoni.

Ardpairin J, Subkrasae C, Dumidae A, Pansri S, Homkaew C, Meesil W Parasit Vectors. 2024; 17(1):529.

PMID: 39710701 PMC: 11665110. DOI: 10.1186/s13071-024-06605-x.

Genome mining reveals novel biosynthetic gene clusters in entomopathogenic bacteria.

Meesil W, Muangpat P, Sitthisak S, Rattanarojpong T, Chantratita N, Machado R Sci Rep. 2023; 13(1):20764.

PMID: 38007490 PMC: 10676414. DOI: 10.1038/s41598-023-47121-9.

RNAi-mediated CHS-2 silencing affects the synthesis of chitin and the formation of the peritrophic membrane in the midgut of Aedes albopictus larvae.

Zhang C, Ding Y, Zhou M, Tang Y, Chen R, Chen Y Parasit Vectors. 2023; 16(1):259.

PMID: 37533099 PMC: 10394979. DOI: 10.1186/s13071-023-05865-3.

Entomopathogenic Nematodes and Their Symbiotic Bacteria from the National Parks of Thailand and Larvicidal Property of Symbiotic Bacteria against and .

Thanwisai A, Muangpat P, Meesil W, Janthu P, Dumidae A, Subkrasae C Biology (Basel). 2022; 11(11).

PMID: 36421372 PMC: 9687835. DOI: 10.3390/biology11111658.

Identification and environment-friendly biocontrol potential of five different bacteria against and (Hemiptera: Aphididae).

Baazeem A, Alotaibi S, Khalaf L, Kumar U, Zaynab M, Alharthi S Front Microbiol. 2022; 13:961349.

PMID: 36386662 PMC: 9640465. DOI: 10.3389/fmicb.2022.961349.

References

Maneesakorn P, An R, Daneshvar H, Taylor K, Bai X, Adams B . Phylogenetic and cophylogenetic relationships of entomopathogenic nematodes (Heterorhabditis: Rhabditida) and their symbiotic bacteria (Photorhabdus: Enterobacteriaceae). Mol Phylogenet Evol. 2011; 59(2):271-80. DOI: 10.1016/j.ympev.2011.02.012. View

Rohde C, Moino Jr A, da Silva M, Carvalho F, Ferreira C . Influence of soil temperature and moisture on the infectivity of entomopathogenic nematodes (Rhabditida: Heterorhabditidae, Steinernematidae) against larvae of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Neotrop Entomol. 2010; 39(4):608-11. DOI: 10.1590/s1519-566x2010000400022. View

Bhatt S, Gething P, Brady O, Messina J, Farlow A, Moyes C . The global distribution and burden of dengue. Nature. 2013; 496(7446):504-7. PMC: 3651993. DOI: 10.1038/nature12060. View

Mracek Z, Qi-Zhi L, Nguyen K . Steinernema xueshanense n. sp. (Rhabditida, Steinernematidae), a new species of entomopathogenic nematode from the province of Yunnan, southeast Tibetan Mts., China. J Invertebr Pathol. 2009; 102(1):69-78. DOI: 10.1016/j.jip.2009.05.006. View

Silva J, Mendes J . Susceptibility of Aedes aegypti (L) to the insect growth regulators diflubenzuron and methoprene in Uberlândia, State of Minas Gerais. Rev Soc Bras Med Trop. 2008; 40(6):612-6. DOI: 10.1590/s0037-86822007000600002. View

Vitta A, Fukruksa C, Yimthin T, Deelue K, Sarai C, Polseela R . PRELIMINARY SURVEY OF ENTOMOPATHOGENIC NEMATODES IN UPPER NORTHERN THAILAND. Southeast Asian J Trop Med Public Health. 2018; 48(1):18-26. View

Salokhe S, Deshpande S, Mukherjee S . Evaluation of the insect growth regulator Lufenuron (Match®) for control of Aedes aegypti by simulated field trials. Parasitol Res. 2012; 111(3):1325-9. DOI: 10.1007/s00436-012-2968-9. View

Dillman A, Guillermin M, Lee J, Kim B, Sternberg P, Hallem E . Olfaction shapes host-parasite interactions in parasitic nematodes. Proc Natl Acad Sci U S A. 2012; 109(35):E2324-33. PMC: 3435218. DOI: 10.1073/pnas.1211436109. View

Tailliez P, Pages S, Ginibre N, Boemare N . New insight into diversity in the genus Xenorhabdus, including the description of ten novel species. Int J Syst Evol Microbiol. 2006; 56(Pt 12):2805-2818. DOI: 10.1099/ijs.0.64287-0. View

10.

Mint Mohamed Lemine A, Ould Lemrabott M, Ebou M, Mint Lekweiry K, Ould Ahmedou Salem M, Ould Brahim K . Mosquitoes (Diptera: Culicidae) in Mauritania: a review of their biodiversity, distribution and medical importance. Parasit Vectors. 2017; 10(1):35. PMC: 5248481. DOI: 10.1186/s13071-017-1978-y. View

11.

Park , Kim . Eicosanoids rescue Spodoptera exigua infected with Xenorhabdus nematophilus, the symbiotic bacteria to the entomopathogenic nematode Steinernema carpocapsae. J Insect Physiol. 2000; 46(11):1469-1476. DOI: 10.1016/s0022-1910(00)00071-8. View

12.

da Silva O, Prado G, Luiz Rosa da Silva J, Silva C, da Costa M, Heermann R . Oral toxicity of Photorhabdus luminescens and Xenorhabdus nematophila (Enterobacteriaceae) against Aedes aegypti (Diptera: Culicidae). Parasitol Res. 2013; 112(8):2891-6. DOI: 10.1007/s00436-013-3460-x. View

13.

Grundmann F, Kaiser M, Schiell M, Batzer A, Kurz M, Thanwisai A . Antiparasitic chaiyaphumines from entomopathogenic Xenorhabdus sp. PB61.4. J Nat Prod. 2014; 77(4):779-83. DOI: 10.1021/np4007525. View

14.

Merritt R, Dadd R, Walker E . Feeding behavior, natural food, and nutritional relationships of larval mosquitoes. Annu Rev Entomol. 1992; 37:349-76. DOI: 10.1146/annurev.en.37.010192.002025. View

15.

Villabona-Arenas C, Zanotto P . Worldwide spread of Dengue virus type 1. PLoS One. 2013; 8(5):e62649. PMC: 3652851. DOI: 10.1371/journal.pone.0062649. View

16.

Ahantarig A, Chantawat N, Waterfield N, Ffrench-Constant R, Kittayapong P . PirAB toxin from Photorhabdus asymbiotica as a larvicide against dengue vectors. Appl Environ Microbiol. 2009; 75(13):4627-9. PMC: 2704829. DOI: 10.1128/AEM.00221-09. View

17.

Puza V, Nermut J, Mracek Z, Gengler S, Haukeland S . Steinernema pwaniensis n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Tanzania. J Helminthol. 2016; 91(1):20-34. DOI: 10.1017/S0022149X15001157. View

18.

Molyneux A . Heterorhabditis spp. and Steinernema (= Neoaplectana) spp.: temperature, and aspects of behavior and infectivity. Exp Parasitol. 1986; 62(2):169-80. DOI: 10.1016/0014-4894(86)90021-4. View

19.

Naqqash M, Gokce A, Bakhsh A, Salim M . Insecticide resistance and its molecular basis in urban insect pests. Parasitol Res. 2016; 115(4):1363-73. DOI: 10.1007/s00436-015-4898-9. View

20.

Cimen H, Lee M, Hatting J, Hazir S, Stock S . Steinernema innovationi n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode species from South Africa. J Helminthol. 2014; 89(4):415-27. DOI: 10.1017/S0022149X14000182. View