Natural Products from Photorhabdus and Xenorhabdus: Mechanisms and Impacts

Overview

Journal Appl Microbiol Biotechnol

Specialties Biomedical Engineering
Biotechnology
Microbiology

Date 2022 Jun 20

PMID 35723692

Authors

Harun Cimen

Mustapha Touray

Sebnem Hazal Gulsen

Selcuk Hazir

Affiliations

Soon will be listed here.

Abstract

Insects and fungal pathogens pose constant problems to public health and agriculture, especially in resource-limited parts of the world; and the use of chemical pesticides continues to be the main methods for the control of these organisms. Photorhabdus spp. and Xenorhabdus spp., (Fam; Morganellaceae), enteric symbionts of Steinernema, and Heterorhabditis nematodes are naturally found in soil on all continents, except Antarctic, and on many islands throughout the world. These bacteria produce diverse secondary metabolites that have important biological and ecological functions. Secondary metabolites include non-ribosomal peptides, polyketides, and/or hybrid natural products that are synthesized using polyketide synthetase (PRS), non-ribosomal peptide synthetase (NRPS), or similar enzymes and are sources of new pesticide/drug compounds and/or can serve as lead molecules for the design and synthesize of new alternatives that could replace current ones. This review addresses the effects of these bacterial symbionts on insect pests, fungal phytopathogens, and animal pathogens and discusses the substances, mechanisms, and impacts on agriculture and public health. KEY POINTS: • Insects and fungi are a constant menace to agricultural and public health. • Chemical-based control results in resistance development. • Photorhabdus and Xenorhabdus are compelling sources of biopesticides.

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References

Abebew D, Sayedain F, Bode E, Bode H . Uncovering Nematicidal Natural Products from Bacteria. J Agric Food Chem. 2022; 70(2):498-506. PMC: 8778618. DOI: 10.1021/acs.jafc.1c05454. View

Adams T, Cohen S, DOULL J, Feron V, Goodman J, Marnett L . The FEMA GRAS assessment of benzyl derivatives used as flavor ingredients. Food Chem Toxicol. 2005; 43(8):1207-40. DOI: 10.1016/j.fct.2004.11.014. View

Ahantarig A, Chantawat N, Waterfield N, Ffrench-Constant R, Kittayapong P . PirAB toxin from Photorhabdus asymbiotica as a larvicide against dengue vectors. Appl Environ Microbiol. 2009; 75(13):4627-9. PMC: 2704829. DOI: 10.1128/AEM.00221-09. View

Almeida F, Rodrigues M, Coelho C . The Still Underestimated Problem of Fungal Diseases Worldwide. Front Microbiol. 2019; 10:214. PMC: 6379264. DOI: 10.3389/fmicb.2019.00214. View

Andersen A . Final report on the safety assessment of benzaldehyde. Int J Toxicol. 2006; 25 Suppl 1:11-27. DOI: 10.1080/10915810600716612. View

Bickers D, Calow P, Greim H, Hanifin J, Rogers A, Saurat J . A toxicologic and dermatologic assessment of cinnamyl alcohol, cinnamaldehyde and cinnamic acid when used as fragrance ingredients. Food Chem Toxicol. 2005; 43(6):799-836. DOI: 10.1016/j.fct.2004.09.013. View

Bode E, Brachmann A, Kegler C, Simsek R, Dauth C, Zhou Q . Simple "on-demand" production of bioactive natural products. Chembiochem. 2015; 16(7):1115-9. DOI: 10.1002/cbic.201500094. View

Booysen E, Dicks L . Does the Future of Antibiotics Lie in Secondary Metabolites Produced by Xenorhabdus spp.? A Review. Probiotics Antimicrob Proteins. 2020; 12(4):1310-1320. DOI: 10.1007/s12602-020-09688-x. View

BOWEN , Ensign . Purification and characterization of a high-molecular-weight insecticidal protein complex produced by the entomopathogenic bacterium photorhabdus luminescens . Appl Environ Microbiol. 1998; 64(8):3029-35. PMC: 106811. DOI: 10.1128/AEM.64.8.3029-3035.1998. View

10.

Bowen D, Rocheleau T, Blackburn M, Andreev O, Golubeva E, Bhartia R . Insecticidal toxins from the bacterium Photorhabdus luminescens. Science. 1998; 280(5372):2129-32. DOI: 10.1126/science.280.5372.2129. View

11.

Cai X, Nowak S, Wesche F, Bischoff I, Kaiser M, Furst R . Entomopathogenic bacteria use multiple mechanisms for bioactive peptide library design. Nat Chem. 2017; 9(4):379-386. DOI: 10.1038/nchem.2671. View

12.

Challinor V, Bode H . Bioactive natural products from novel microbial sources. Ann N Y Acad Sci. 2015; 1354:82-97. DOI: 10.1111/nyas.12954. View

13.

Chang D, Serra L, Lu D, Mortazavi A, Dillman A . A core set of venom proteins is released by entomopathogenic nematodes in the genus Steinernema. PLoS Pathog. 2019; 15(5):e1007626. PMC: 6513111. DOI: 10.1371/journal.ppat.1007626. View

14.

Cheng S, Liu J, Huang C, Hsui Y, Chen W, Chang S . Insecticidal activities of leaf essential oils from Cinnamomum osmophloeum against three mosquito species. Bioresour Technol. 2008; 100(1):457-64. DOI: 10.1016/j.biortech.2008.02.030. View

15.

Cimen H, Touray M, Hazal Gulsen S, Erincik O, Wenski S, Bode H . Antifungal activity of different Xenorhabdus and Photorhabdus species against various fungal phytopathogens and identification of the antifungal compounds from X. szentirmaii. Appl Microbiol Biotechnol. 2021; 105(13):5517-5528. DOI: 10.1007/s00253-021-11435-3. View

16.

Daborn P, Waterfield N, Silva C, Au C, Sharma S, Ffrench-Constant R . A single Photorhabdus gene, makes caterpillars floppy (mcf), allows Escherichia coli to persist within and kill insects. Proc Natl Acad Sci U S A. 2002; 99(16):10742-7. PMC: 125031. DOI: 10.1073/pnas.102068099. View

17.

Ozkan H, Cimen H, Ulug D, Wenski S, Ozer S, Telli M . Nematode-Associated Bacteria: Production of Antimicrobial Agent as a Presumptive Nominee for Curing Endodontic Infections Caused by . Front Microbiol. 2019; 10:2672. PMC: 6882856. DOI: 10.3389/fmicb.2019.02672. View

18.

Eom S, Park Y, Kim H, Kim Y . Development of a high efficient "Dual Bt-Plus" insecticide using a primary form of an entomopathogenic bacterium, Xenorhabdus nematophila. J Microbiol Biotechnol. 2014; 24(4):507-21. DOI: 10.4014/jmb.1310.10116. View

19.

Fang X, Li Z, Wang Y, Zhang X . In vitro and in vivo antimicrobial activity of Xenorhabdus bovienii YL002 against Phytophthora capsici and Botrytis cinerea. J Appl Microbiol. 2011; 111(1):145-54. DOI: 10.1111/j.1365-2672.2011.05033.x. View

20.

Fisher M, Hawkins N, Sanglard D, Gurr S . Worldwide emergence of resistance to antifungal drugs challenges human health and food security. Science. 2018; 360(6390):739-742. DOI: 10.1126/science.aap7999. View