FIGL1 and Its Novel Partner FLIP Form a Conserved Complex That Regulates Homologous Recombination

Overview

Journal PLoS Genet

Specialty Genetics

Date 2018 Apr 3

PMID 29608566

Citations 54

Authors

Joiselle Blanche Fernandes

Marine Duhamel

Mathilde Seguela-Arnaud

Nicole Froger

Chloe Girard

Sandrine Choinard

Victor Solier

Nancy De Winne

Geert De Jaeger

Kris Gevaert

Philippe Andrey

Mathilde Grelon

Raphael Guerois

Rajeev Kumar

Raphael Mercier

Affiliations

Soon will be listed here.

Abstract

Homologous recombination is central to repair DNA double-strand breaks, either accidently arising in mitotic cells or in a programed manner at meiosis. Crossovers resulting from the repair of meiotic breaks are essential for proper chromosome segregation and increase genetic diversity of the progeny. However, mechanisms regulating crossover formation remain elusive. Here, we identified through genetic and protein-protein interaction screens FIDGETIN-LIKE-1 INTERACTING PROTEIN (FLIP) as a new partner of the previously characterized anti-crossover factor FIDGETIN-LIKE-1 (FIGL1) in Arabidopsis thaliana. We showed that FLIP limits meiotic crossover together with FIGL1. Further, FLIP and FIGL1 form a protein complex conserved from Arabidopsis to human. FIGL1 interacts with the recombinases RAD51 and DMC1, the enzymes that catalyze the DNA strand exchange step of homologous recombination. Arabidopsis flip mutants recapitulate the figl1 phenotype, with enhanced meiotic recombination associated with change in counts of DMC1 and RAD51 foci. Our data thus suggests that FLIP and FIGL1 form a conserved complex that regulates the crucial step of strand invasion in homologous recombination.

Citing Articles

Molecular basis of FIGNL1 in dissociating RAD51 from DNA and chromatin.

Carver A, Yu T, Yates L, White T, Wang R, Lister K Science. 2024; 387(6732):426-431.

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Genome-wide atlas of loci involved in chromosomal recombination in common wheat.

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FIGL1 attenuates meiotic interhomolog repair and is counteracted by the RAD51 paralog XRCC2 and the chromosome axis protein ASY1 during meiosis.

Emmenecker C, Pakzad S, Ture F, Guerin J, Hurel A, Chambon A New Phytol. 2024; 244(6):2442-2457.

PMID: 39420761 PMC: 11579446. DOI: 10.1111/nph.20181.

FIGNL1-FIRRM is essential for meiotic recombination and prevents DNA damage-independent RAD51 and DMC1 loading.

Zainu A, Dupaigne P, Bouchouika S, Cau J, Clement J, Auffret P Nat Commun. 2024; 15(1):7015.

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Molecular basis of FIGNL1 in dissociating RAD51 from DNA and chromatin.

Carver A, Yu T, Yates L, White T, Wang R, Lister K bioRxiv. 2024; .

PMID: 39071279 PMC: 11275795. DOI: 10.1101/2024.07.16.603765.

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