Protective Effect of Quercetin in LPS-Induced Murine Acute Lung Injury Mediated by CAMP-Epac Pathway

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2018 Mar 24

PMID 29569077

Citations 17

Authors

Xue-Feng Wang

Shun-de Song

Ya-Jun Li

Zheng Qiang Hu

Zhe-Wen Zhang

Chun-Guang Yan

Zi-Gang Li

Hui-Fang Tang

Affiliations

Soon will be listed here.

Abstract

Quercetin (Que) as an abundant flavonol element possesses potent antioxidative properties and has protective effect in lipopolysaccharide (LPS)-induced acute lung injury (ALI), but the specific mechanism is still unclear, so we investigated the effect of Que from in vivo and in vitro studies and the related mechanism of cAMP-PKA/Epac pathway. The results in mice suggested that Que can inhibit the release of inflammatory cytokine, block neutrophil recruitment, and decrease the albumin leakage in dose-dependent manners. At the same time, Que can increase the cAMP content of lung tissue, and Epac content, except PKA. The results in epithelial cell (MLE-12) suggested that Que also can inhibit the inflammatory mediators keratinocyte-derived chemokines release after LPS stimulation; Epac inhibitor ESI-09 functionally antagonizes the inhibitory effect of Que; meanwhile, PKA inhibitor H89 functionally enhances the inhibitory effect of Que. Overexpression of Epac1 in MLE-12 suggested that Epac1 enhance the effect of Que. All those results suggested that the protective effect of quercetin in ALI is involved in cAMP-Epac pathway.

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