Determination of Mitochondrial Oxygen Consumption in the Retina Ex Vivo: Applications for Retinal Disease

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2018 Mar 23

PMID 29564788

Citations 2

Authors

Yogita K Adlakha

Anand Swaroop

Affiliations

Soon will be listed here.

Abstract

Mitochondrial dysfunction, and consequently altered aerobic energy metabolism, is associated with numerous retinal diseases, including photoreceptor degeneration and diabetic retinopathy. Here, we describe a detailed protocol to directly measure oxygen consumption in the intact retina ex vivo using microplate-based fluorescence technology. We have used this method to assess preferred energy substrate for retinal tissue and suggested its application for investigating mechanisms of retinal disease.

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