Characterization of an Integrated Active Glu-1Ay Allele in Common Wheat from Wild Emmer and Its Potential Role in Flour Improvement

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2018 Mar 22

PMID 29561750

Citations 10

Authors

Zhenzhen Wang

Lin Huang

Bihua Wu

Jiliang Hu

Zilong Jiang

Pengfei Qi

Youliang Zheng

Dengcai Liu

Affiliations

Soon will be listed here.

Abstract

, one of six genes encoding a high molecular weight glutenin subunit (HMW-GS), is frequently silenced in hexaploid common wheat. Here, an active allele of was integrated from wild emmer wheat ( ssp. ) accession D97 into the common wheat () cultivar Chuannong 16 via the repeated self-fertilization of the pentaploid interspecific hybrid, culminating in the selection of a line TaAy7-40 shown to express the wild emmer allele. The open reading frame of this allele was a 1830 bp long sequence, demonstrated by its heterologous expression in to encode a 608-residue polypeptide. Its nucleotide sequence was 99.2% identical to that of the sequence within the wild emmer parent. The TaAy7-40 introgression line containing the active allele showed higher protein content, higher sodium dodecyl sulfate (SDS) sedimentation value, higher content of wet gluten in the flour, higher grain weight, and bigger grain size than Chuannong 16. The end-use quality parameters of the TaAy7-40 were superior to those of the medium gluten common wheat cultivars Mianmai 37 and Neimai 9. Thus, the active allele might be of potential value in breeding programs designed to improve wheat flour quality.

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References

Shewry P, Halford N . Cereal seed storage proteins: structures, properties and role in grain utilization. J Exp Bot. 2002; 53(370):947-58. DOI: 10.1093/jexbot/53.370.947. View

Wan Y, Wang D, Shewry R, Halford G . Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet. 2003; 104(5):828-839. DOI: 10.1007/s00122-001-0793-0. View

Shewry P, Gilbert S, Savage A, Tatham A, Wan Y, Belton P . Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum) which is associated with poor end use properties. Theor Appl Genet. 2003; 106(4):744-50. DOI: 10.1007/s00122-002-1135-6. View

Shewry P, Halford N, Lafiandra D . Genetics of wheat gluten proteins. Adv Genet. 2003; 49:111-84. DOI: 10.1016/s0065-2660(03)01003-4. View

Xu S, Khan K, Klindworth D, Faris J, Nygard G . Chromosomal location of genes for novel glutenin subunits and gliadins in wild emmer wheat (Triticum turgidum L. var. dicoccoides). Theor Appl Genet. 2004; 108(7):1221-8. DOI: 10.1007/s00122-003-1555-y. View

McCLINTOCK B . The significance of responses of the genome to challenge. Science. 1984; 226(4676):792-801. DOI: 10.1126/science.15739260. View

Harberd N, Flavell R, Thompson R . Identification of a transposon-like insertion in a Glu-1 allele of wheat. Mol Gen Genet. 1987; 209(2):326-32. DOI: 10.1007/BF00329661. View

Pang B, Zhang X . Isolation and molecular characterization of high molecular weight glutenin subunit genes 1Bx13 and 1By16 from hexaploid wheat. J Integr Plant Biol. 2008; 50(3):329-37. DOI: 10.1111/j.1744-7909.2007.00573.x. View

Jiang Q, Wei Y, Wang F, Wang J, Yan Z, Zheng Y . Characterization and comparative analysis of HMW glutenin 1Ay alleles with differential expressions. BMC Plant Biol. 2009; 9:16. PMC: 2667398. DOI: 10.1186/1471-2229-9-16. View

10.

Hua W, Liu Z, Zhu J, Xie C, Yang T, Zhou Y . Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet. 2009; 119(2):223-30. DOI: 10.1007/s00122-009-1031-4. View

11.

Dumur J, Branlard G, Tanguy A, Dardevet M, Coriton O, Huteau V . Development of isohomoeoallelic lines within the wheat cv. Courtot for high molecular weight glutenin subunits: transfer of the Glu-D1 locus to chromosome 1A. Theor Appl Genet. 2009; 119(3):471-81. DOI: 10.1007/s00122-009-1053-y. View

12.

Dumur J, Branlard G, Tanguy A, Dardevet M, Coriton O, Huteau V . Homoeologous recombination within bread wheat to develop novel combinations of HMW-GS genes: transfer of the Glu-A1 locus to chromosome 1D. Planta. 2009; 231(1):57-65. DOI: 10.1007/s00425-009-1027-y. View

13.

Gao L, Ma W, Chen J, Wang K, Li J, Wang S . Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS. J Agric Food Chem. 2010; 58(5):2777-86. DOI: 10.1021/jf903363z. View

14.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

15.

Guo X, Wu B, Hu X, Bi Z, Wang Z, Liu D . Molecular characterization of two y-type high molecular weight glutenin subunit alleles 1Ay12 and 1Ay8 from cultivated einkorn wheat (Triticum monococcum ssp. monococcum). Gene. 2012; 516(1):1-7. DOI: 10.1016/j.gene.2012.12.037. View

16.

Guo X, Bi Z, Wu B, Wang Z, Hu J, Zheng Y . ChAy/Bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in Triticum turgidum ssp. dicoccoides. Gene. 2013; 531(2):318-25. DOI: 10.1016/j.gene.2013.08.073. View

17.

Nevo E, Pagnotta M, Beiles A, Porceddu E . Wheat storage proteins: glutenin DNA diversity in wild emmer wheat, Triticum dicoccoids, in Israel and Turkey. 3. Environmental correlates and allozymic associations. Theor Appl Genet. 2013; 91(3):415-20. DOI: 10.1007/BF00222968. View

18.

Zhang H, Bian Y, Gou X, Dong Y, Rustgi S, Zhang B . Intrinsic karyotype stability and gene copy number variations may have laid the foundation for tetraploid wheat formation. Proc Natl Acad Sci U S A. 2013; 110(48):19466-71. PMC: 3845155. DOI: 10.1073/pnas.1319598110. View

19.

Carrillo J, Rousset M, Qualset C, Kasarda D . Use of recombinant inbred lines of wheat for study of associations of high-molecular-weight glutenin subunit alleles to quantitative traits : 1. Grain yield and quality prediction tests. Theor Appl Genet. 2013; 79(3):321-30. DOI: 10.1007/BF01186074. View

20.

Waines J, Payne P . Electrophoretic analysis of the high-molecular-weight glutenin subunits of Triticum monococcum, T. urartu, and the A genome of bread wheat (T. aestivum). Theor Appl Genet. 2013; 74(1):71-6. DOI: 10.1007/BF00290086. View