The Effect of Storage Delay and Storage Temperature on Orthopaedic Surgical Samples Contaminated by Staphylococcus Epidermidis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 Mar 20

PMID 29554102

Citations 6

Authors

Maite Van Cauter

Olivier Cornu

Jean-Cyr Yombi

Hector Rodriguez-Villalobos

Ludovic Kaminski

Affiliations

Soon will be listed here.

Abstract

Background: Prosthetic Joint Infection (PJI) is a rare but devastating complications with high morbitity and mortality. The identification of the causal microorganism remains crucial and determines therapeutic strategies and success. Microbiology cultures remain the common method to diagnose PJI. Unfortunately, 14% of intra-articular punctures remain negative after culture. The microorganisms are best detected by inoculation of microbiology samples in blood culture bottles (Bactec), or after sonication of the implant and polymerase chain reaction (PCR). The identification of the causal microorganism remains crucial and determines therapeutic success.

Objectives: This study was conducted to assess the effect of culture lead time and sample storage temperature on the detection of the pathogen.

Methods: We obtained bone fragments from femoral heads during primary arthroplasty. Bone fragments were contaminated with a strain of Staphylococcus epidermidis. Four set-ups with different combinations of storage delay and storage temperature were tested.

Results: Our study shows the need to cultivate as soon as possible and optimally within 2h after the completion of sampling. Temporary storage in a refrigerator at 4°C also appears to have a positive influence on bacterial viability. At present, these conclusions concern only the Staphylococcus Epidermidis. Others studies are requested to generalize this conclusion to other bacteria.

Citing Articles

Can pre-analytical procedures improve microbiological culture yield in patients with periprosthetic infections?.

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Improved cure rate of periprosthetic joint infection through targeted antibiotic therapy based on integrated pathogen diagnosis strategy.

Wang Q, Chen Y, Chen Y, Lv J, Ding H, Huang J Front Cell Infect Microbiol. 2024; 14:1388385.

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Culture-negative periprosthetic joint infections: Do we have an issue?.

Tsai S, Mu W, Parvizi J J Clin Orthop Trauma. 2024; 52:102430.

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Cultures in periprosthetic joint infections, the imperfect gold standard?.

Wouthuyzen-Bakker M EFORT Open Rev. 2023; 8(4):175-179.

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