Caenorhabditis Elegans Sperm Membrane Protein Interactome

Overview

Journal Biol Reprod

Publisher Oxford University Press

Specialty Reproductive Medicine

Date 2018 Mar 17

PMID 29546388

Citations 8

Authors

Matthew R Marcello

Marina Druzhinina

Andrew Singson

Affiliations

Soon will be listed here.

Abstract

The interaction and organization of proteins in the sperm membrane are important for all aspects of sperm function. We have determined the interactions between 12 known mutationally defined and cloned sperm membrane proteins in a model system for reproduction, the nematode Caenorhabditis elegans. Identification of the interactions between sperm membrane proteins will improve our understanding of and ability to characterize defects in sperm function. To identify interacting proteins, we conducted a split-ubiquitin membrane yeast two-hybrid analysis of gene products identified through genetic screens that are necessary for sperm function and predicted to encode transmembrane proteins. Our analysis revealed novel interactions between sperm membrane proteins known to have roles in spermatogenesis, spermiogenesis, and fertilization. For example, we found that a protein known to play a role in sperm function during fertilization, SPE-38 (a predicted four pass transmembrane protein), interacts with proteins necessary for spermiogenesis and spermatogenesis and could serve as a central organizing protein in the plasma membrane. These novel interaction pairings will provide the foundation for investigating previously unrealized membrane protein interactions during spermatogenesis, spermiogenesis, and sperm function during fertilization.

Citing Articles

SPE-51, a sperm-secreted protein with an immunoglobulin-like domain, is required for fertilization in C. elegans.

Mei X, Maniates K, Looper A, Krauchunas A, Druzhinina M, Dharia S Curr Biol. 2023; 33(14):3048-3055.e6.

PMID: 37453427 PMC: 10528068. DOI: 10.1016/j.cub.2023.06.029.

Oogenesis in Caenorhabditis elegans.

Davis G, Hipwell H, Boag P Sex Dev. 2023; 17(2-3):73-83.

PMID: 37232019 PMC: 10659005. DOI: 10.1159/000531019.

CRISPR/Cas9 Mediated Fluorescent Tagging of SPE-38 Reveals a Complete Localization Pattern in Live Spermatozoa.

Zuo Y, Mei X, Singson A Biomolecules. 2023; 13(4).

PMID: 37189371 PMC: 10136291. DOI: 10.3390/biom13040623.

The molecular underpinnings of fertility: Genetic approaches in .

Mei X, Singson A Adv Genet (Hoboken). 2021; 2(1).

PMID: 34322672 PMC: 8315475. DOI: 10.1002/ggn2.10034.

Conserved sperm factors are no longer a bone of contention.

Mei X, Singson A Elife. 2021; 10.

PMID: 33908866 PMC: 8081520. DOI: 10.7554/eLife.68976.

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