Different Chondrogenic Potential Among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2018 Mar 15

PMID 29535785

Citations 7

Authors

Yeri Alice Rim

Yoojun Nam

Narae Park

Hyerin Jung

Yeonsue Jang

Jennifer Lee

Ji Hyeon Ju

Affiliations

Soon will be listed here.

Abstract

Scientists have tried to reprogram various origins of primary cells into human induced pluripotent stem cells (hiPSCs). Every somatic cell can theoretically become a hiPSC and give rise to targeted cells of the human body. However, there have been debates on the controversy about the differentiation propensity according to the origin of primary cells. We reprogrammed hiPSCs from four different types of primary cells such as dermal fibroblasts (DF, = 3), peripheral blood mononuclear cells (PBMC, = 3), cord blood mononuclear cells (CBMC, = 3), and osteoarthritis fibroblast-like synoviocytes (OAFLS, = 3). Established hiPSCs were differentiated into chondrogenic pellets. All told, cartilage-specific markers tended to express more by the order of CBMC > DF > PBMC > FLS. Origin of primary cells may influence the reprogramming and differentiation thereafter. In the context of chondrogenic propensity, CBMC-derived hiPSCs can be a fairly good candidate cell source for cartilage regeneration. The differentiation of hiPSCs into chondrocytes may help develop "cartilage in a dish" in the future. Also, the ideal cell source of hiPSC for chondrogenesis may contribute to future application as well.

Citing Articles

Cell Reprogramming Strategies for Treating Osteoarthritis and Intervertebral Disc Degeneration.

Jiang Z, Cao C, Zhang Y, Yan M, Song Z, Shang G Aging Dis. 2024; .

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Differential chondrogenic differentiation between iPSC derived from healthy and OA cartilage is associated with changes in epigenetic regulation and metabolic transcriptomic signatures.

Khan N, Diaz-Hernandez M, Chihab S, Priyadarshani P, Bhattaram P, Mortensen L Elife. 2023; 12.

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The Induced Pluripotent Stem Cells in Articular Cartilage Regeneration and Disease Modelling: Are We Ready for Their Clinical Use?.

Lach M, Rosochowicz M, Richter M, Jagiello I, Suchorska W, Trzeciak T Cells. 2022; 11(3).

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Insights into the present and future of cartilage regeneration and joint repair.

Evenbratt H, Andreasson L, Bicknell V, Brittberg M, Mobini R, Simonsson S Cell Regen. 2022; 11(1):3.

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Lupus Heart Disease Modeling with Combination of Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Lupus Patient Serum.

Park N, Rim Y, Jung H, Nam Y, Ju J Int J Stem Cells. 2021; 15(3):233-246.

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