Longitudinal Surveillance of Betacoronaviruses in Fruit Bats in Yunnan Province, China During 2009-2016

Overview

Journal Virol Sin

Specialty Microbiology

Date 2018 Mar 4

PMID 29500692

Citations 18

Authors

Yun Luo

Bei Li

Ren-Di Jiang

Bing-Jie Hu

Dong-Sheng Luo

Guang-Jian Zhu

Ben Hu

Hai-Zhou Liu

Yun-Zhi Zhang

Xing-Lou Yang

Zheng-Li Shi

Affiliations

Soon will be listed here.

Abstract

Previous studies indicated that fruit bats carry two betacoronaviruses, BatCoV HKU9 and BatCoV GCCDC1. To investigate the epidemiology and genetic diversity of these coronaviruses, we conducted a longitudinal surveillance in fruit bats in Yunnan province, China during 2009-2016. A total of 59 (10.63%) bat samples were positive for the two betacorona-viruses, 46 (8.29%) for HKU9 and 13 (2.34%) for GCCDC1, or closely related viruses. We identified a novel HKU9 strain, tentatively designated as BatCoV HKU9-2202, by sequencing the full-length genome. The BatCoV HKU9-2202 shared 83% nucleotide identity with other BatCoV HKU9 stains based on whole genome sequences. The most divergent region is in the spike protein, which only shares 68% amino acid identity with BatCoV HKU9. Quantitative PCR revealed that the intestine was the primary infection organ of BatCoV HKU9 and GCCDC1, but some HKU9 was also detected in the heart, kidney, and lung tissues of bats. This study highlights the importance of virus surveillance in natural reservoirs and emphasizes the need for preparedness against the potential spill-over of these viruses to local residents living near bat caves.

Citing Articles

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References

Hu B, Ge X, Wang L, Shi Z . Bat origin of human coronaviruses. Virol J. 2015; 12:221. PMC: 4687304. DOI: 10.1186/s12985-015-0422-1. View

Drosten C, Gunther S, Preiser W, van der Werf S, Brodt H, Becker S . Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med. 2003; 348(20):1967-76. DOI: 10.1056/NEJMoa030747. View

He B, Feng Y, Zhang H, Xu L, Yang W, Zhang Y . Filovirus RNA in Fruit Bats, China. Emerg Infect Dis. 2015; 21(9):1675-7. PMC: 4550138. DOI: 10.3201/eid2109.150260. View

Hunter P . The nature of flight. The molecules and mechanics of flight in animals. EMBO Rep. 2007; 8(9):811-3. PMC: 1973956. DOI: 10.1038/sj.embor.7401050. View

Stuckey M, Chomel B, de Fleurieu E, Aguilar-Setien A, Boulouis H, Chang C . Bartonella, bats and bugs: A review. Comp Immunol Microbiol Infect Dis. 2017; 55:20-29. DOI: 10.1016/j.cimid.2017.09.001. View

Du L, Lu Z, Fan Y, Meng K, Jiang Y, Zhu Y . Xi River virus, a new bat reovirus isolated in southern China. Arch Virol. 2010; 155(8):1295-9. PMC: 7087202. DOI: 10.1007/s00705-010-0690-4. View

Li Y, Wang J, Hickey A, Zhang Y, Li Y, Wu Y . Antibodies to Nipah or Nipah-like viruses in bats, China. Emerg Infect Dis. 2008; 14(12):1974-6. PMC: 2634619. DOI: 10.3201/eid1412.080359. View

Agnarsson I, Zambrana-Torrelio C, Flores-Saldana N, May-Collado L . A time-calibrated species-level phylogeny of bats (Chiroptera, Mammalia). PLoS Curr. 2011; 3:RRN1212. PMC: 3038382. DOI: 10.1371/currents.RRN1212. View

Channappanavar R, Perlman S . Pathogenic human coronavirus infections: causes and consequences of cytokine storm and immunopathology. Semin Immunopathol. 2017; 39(5):529-539. PMC: 7079893. DOI: 10.1007/s00281-017-0629-x. View

10.

Tan B, Yang X, Ge X, Peng C, Liu H, Zhang Y . Novel bat adenoviruses with low G+C content shed new light on the evolution of adenoviruses. J Gen Virol. 2017; 98(4):739-748. DOI: 10.1099/jgv.0.000739. View

11.

Li W, Shi Z, Yu M, Ren W, Smith C, Epstein J . Bats are natural reservoirs of SARS-like coronaviruses. Science. 2005; 310(5748):676-9. DOI: 10.1126/science.1118391. View

12.

Jiang Y, Wang L, Lu Z, Xuan H, Han X, Xia X . Seroprevalence of rabies virus antibodies in bats from southern China. Vector Borne Zoonotic Dis. 2009; 10(2):177-81. DOI: 10.1089/vbz.2008.0212. View

13.

Yang X, Zhang Y, Jiang R, Guo H, Zhang W, Li B . Genetically Diverse Filoviruses in Rousettus and Eonycteris spp. Bats, China, 2009 and 2015. Emerg Infect Dis. 2017; 23(3):482-486. PMC: 5382765. DOI: 10.3201/eid2303.161119. View

14.

Li Z, Liu D, Ran X, Liu C, Guo D, Hu X . Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats. Infect Genet Evol. 2016; 43:347-53. PMC: 7106163. DOI: 10.1016/j.meegid.2016.05.039. View

15.

Huang C, Liu W, Xu W, Jin T, Zhao Y, Song J . A Bat-Derived Putative Cross-Family Recombinant Coronavirus with a Reovirus Gene. PLoS Pathog. 2016; 12(9):e1005883. PMC: 5038965. DOI: 10.1371/journal.ppat.1005883. View

16.

de Souza Luna L, Heiser V, Regamey N, Panning M, Drexler J, Mulangu S . Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray. J Clin Microbiol. 2007; 45(3):1049-52. PMC: 1829107. DOI: 10.1128/JCM.02426-06. View

17.

Shi Z . Emerging infectious diseases associated with bat viruses. Sci China Life Sci. 2013; 56(8):678-82. PMC: 7088756. DOI: 10.1007/s11427-013-4517-x. View

18.

Lau S, Poon R, Wong B, Wang M, Huang Y, Xu H . Coexistence of different genotypes in the same bat and serological characterization of Rousettus bat coronavirus HKU9 belonging to a novel Betacoronavirus subgroup. J Virol. 2010; 84(21):11385-94. PMC: 2953156. DOI: 10.1128/JVI.01121-10. View

19.

de Groot R, Baker S, Baric R, Brown C, Drosten C, Enjuanes L . Middle East respiratory syndrome coronavirus (MERS-CoV): announcement of the Coronavirus Study Group. J Virol. 2013; 87(14):7790-2. PMC: 3700179. DOI: 10.1128/JVI.01244-13. View

20.

Woo P, Wang M, Lau S, Xu H, Poon R, Guo R . Comparative analysis of twelve genomes of three novel group 2c and group 2d coronaviruses reveals unique group and subgroup features. J Virol. 2006; 81(4):1574-85. PMC: 1797546. DOI: 10.1128/JVI.02182-06. View