A Model for the Phosphorylation of the Ca2+ + Mg2+-activated ATPase by Phosphate

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Jul 1

PMID 2948489

Citations 14

Authors

R J Froud

A G Lee

Affiliations

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Abstract

We have shown that changes in fluorescence intensity for the Ca2+ + Mg2+-activated ATPase of sarcoplasmic reticulum labelled with fluorescein isothiocyanate following the addition of Ca2+ can give the ratio of the two conformations (E1 and E2) of the ATPase. We show that the fluorescence response to Ca2+ is unaffected by Mg2+, phosphate or K+, implying that these ions bind equally well to the E1 and E2 conformations. A model is presented for phosphorylation of the ATPase by phosphate as a function of pH, Mg2+, K+ and Ca2+.

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References

Duggan P, Martonosi A . Sarcoplasmic reticulum. IX. The permeability of sarcoplasmic reticulum membranes. J Gen Physiol. 1970; 56(2):147-67. PMC: 2225859. DOI: 10.1085/jgp.56.2.147. View

Gould G, East J, Froud R, McWhirter J, Stefanova H, Lee A . A kinetic model for the Ca2+ + Mg2+-activated ATPase of sarcoplasmic reticulum. Biochem J. 1986; 237(1):217-27. PMC: 1146968. DOI: 10.1042/bj2370217. View

McKinley D, Meissner G . Evidence for a K+, Na+ permeable channel in sarcoplasmic reticulum. J Membr Biol. 1978; 44(2):159-86. DOI: 10.1007/BF01976037. View

Punzengruber C, Prager R, Kolassa N, Winkler F, Suko J . Calcium gradient-dependent and calcium gradient-independent phosphorylation of sarcoplasmic reticulum by orthophosphate. The role of magnesium. Eur J Biochem. 1978; 92(2):349-59. DOI: 10.1111/j.1432-1033.1978.tb12754.x. View

De Meis L, Vianna A . Energy interconversion by the Ca2+-dependent ATPase of the sarcoplasmic reticulum. Annu Rev Biochem. 1979; 48:275-92. DOI: 10.1146/annurev.bi.48.070179.001423. View

Prager R, Punzengruber C, Kolassa N, Winkler F, Suko J . Ionized and bound calcium inside isolated sarcoplasmic reticulum of skeletal muscle and its significance in phosphorylation of adenosine triphosphatase by orthophosphate. Eur J Biochem. 1979; 97(1):239-50. DOI: 10.1111/j.1432-1033.1979.tb13108.x. View

Chaloub R, De Meis L . Effect of K+ on phosphorylation of the sarcoplasmic reticulum ATPase by either Pi or ATP. J Biol Chem. 1980; 255(13):6168-72. View

Chiu V, Haynes D . Rapid kinetic study of the passive permeability of a Ca2+-ATPase rich fraction of the sarcoplasmic reticulum. J Membr Biol. 1980; 56(3):203-18. DOI: 10.1007/BF01869477. View

Martin D, Tanford C . Phosphorylation of calcium adenosinetriphosphatase by inorganic phosphate: van't Hoff analysis of enthalpy changes. Biochemistry. 1981; 20(16):4597-602. DOI: 10.1021/bi00519a013. View

10.

Suko J, Plank B, Preis P, Kolassa N, Hellmann G, Conca W . Formation of magnesium-phosphoenzyme and magnesium-calcium-phosphoenzyme in the phosphorylation of adenosine triphosphatase by orthophosphate in sarcoplasmic reticulum. Models of a reaction sequence. Eur J Biochem. 1981; 119(2):225-36. DOI: 10.1111/j.1432-1033.1981.tb05598.x. View

11.

Loomis C, Martin D, McCaslin D, Tanford C . Phosphorylation of calcium adenosinetriphosphatase by inorganic phosphate: reversible inhibition at high magnesium ion concentrations. Biochemistry. 1982; 21(1):151-6. DOI: 10.1021/bi00530a026. View

12.

De Meis L, de Souza Otero A, Martins O, Alves E, Inesi G, Nakamoto R . Phosphorylation of sarcoplasmic reticulum ATPase by orthophosphate in the absence of Ca2+ gradient. Contribution of water activity to the enthalpy and the entropy changes. J Biol Chem. 1982; 257(9):4993-8. View

13.

Pick U, Karlish S . Regulation of the conformation transition in the Ca-ATPase from sarcoplasmic reticulum by pH, temperature, and calcium ions. J Biol Chem. 1982; 257(11):6120-6. View

14.

Tanford C, Martin D . Equilibrium constants for some steps of the reaction cycle of the sarcoplasmic reticulum calcium pump. Z Naturforsch C Biosci. 1982; 37(5-6):522-6. DOI: 10.1515/znc-1982-5-626. View

15.

McIntosh D, BOYER P . Adenosine 5'-triphosphate modulation of catalytic intermediates of calcium ion activated adenosinetriphosphatase of sarcoplasmic reticulum subsequent to enzyme phosphorylation. Biochemistry. 1983; 22(12):2867-75. DOI: 10.1021/bi00281a015. View

16.

Inesi G, Hill T . Calcium and proton dependence of sarcoplasmic reticulum ATPase. Biophys J. 1983; 44(2):271-80. PMC: 1434830. DOI: 10.1016/S0006-3495(83)84299-4. View

17.

Inesi G, Lewis D, Murphy A . Interdependence of H+, Ca2+, and Pi (or vanadate) sites in sarcoplasmic reticulum ATPase. J Biol Chem. 1984; 259(2):996-1003. View

18.

Pickart C, Jencks W . Energetics of the calcium-transporting ATPase. J Biol Chem. 1984; 259(3):1629-43. View

19.

Gafni A, BOYER P . Characterization of sarcoplasmic reticulum adenosinetriphosphatase purified by selective column adsorption. Biochemistry. 1984; 23(19):4362-7. DOI: 10.1021/bi00314a018. View

20.

Guillain F, Champeil P, BOYER P . Sarcoplasmic reticulum adenosinetriphosphatase phosphorylation from inorganic phosphate. Theoretical and experimental reinvestigation. Biochemistry. 1984; 23(20):4754-61. DOI: 10.1021/bi00315a034. View