Proteomic Profile of Human Spermatozoa in Healthy and Asthenozoospermic Individuals

Overview

Journal Reprod Biol Endocrinol

Publisher Biomed Central

Specialties Endocrinology
Reproductive Medicine

Date 2018 Feb 28

PMID 29482568

Citations 32

Authors

Xiaodan Cao

Yun Cui

Xiaoxia Zhang

Jiangtao Lou

Jun Zhou

Huafeng Bei

Renxiong Wei

Affiliations

Soon will be listed here.

Abstract

Asthenozoospermia is considered as a common cause of male infertility and characterized by reduced sperm motility. However, the molecular mechanism that impairs sperm motility remains unknown in most cases. In the present review, we briefly reviewed the proteome of spermatozoa and seminal plasma in asthenozoospermia and considered post-translational modifications in spermatozoa of asthenozoospermia. The reduction of sperm motility in asthenozoospermic patients had been attributed to factors, for instance, energy metabolism dysfunction or structural defects in the sperm-tail protein components and the differential proteins potentially involved in sperm motility such as COX6B, ODF, TUBB2B were described. Comparative proteomic analysis open a window to discover the potential pathogenic mechanisms of asthenozoospermia and the biomarkers with clinical significance.

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References

Choudhary O, Ujwal R, Kowallis W, Coalson R, Abramson J, Grabe M . The electrostatics of VDAC: implications for selectivity and gating. J Mol Biol. 2009; 396(3):580-92. PMC: 3736979. DOI: 10.1016/j.jmb.2009.12.006. View

Fujinoki M, Kawamura T, Toda T, Ohtake H, Shimizu N, Yamaoka S . Identification of the 58-kDa phosphoprotein associated with motility initiation of hamster spermatozoa. J Biochem. 2003; 134(4):559-65. DOI: 10.1093/jb/mvg174. View

Schneider H, Berthold J, Bauer M, Dietmeier K, Guiard B, Brunner M . Mitochondrial Hsp70/MIM44 complex facilitates protein import. Nature. 1994; 371(6500):768-74. DOI: 10.1038/371768a0. View

Siva A, Kameshwari D, Singh V, Pavani K, Sundaram C, Rangaraj N . Proteomics-based study on asthenozoospermia: differential expression of proteasome alpha complex. Mol Hum Reprod. 2010; 16(7):452-62. DOI: 10.1093/molehr/gaq009. View

Miki K, Willis W, Brown P, Goulding E, Fulcher K, Eddy E . Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility. Dev Biol. 2002; 248(2):331-42. DOI: 10.1006/dbio.2002.0728. View

Eddy E, Toshimori K, OBrien D . Fibrous sheath of mammalian spermatozoa. Microsc Res Tech. 2003; 61(1):103-15. DOI: 10.1002/jemt.10320. View

Brohmann H, Pinnecke S, Hoyer-Fender S . Identification and characterization of new cDNAs encoding outer dense fiber proteins of rat sperm. J Biol Chem. 1997; 272(15):10327-32. DOI: 10.1074/jbc.272.15.10327. View

Anderson N, Anderson N . Proteome and proteomics: new technologies, new concepts, and new words. Electrophoresis. 1998; 19(11):1853-61. DOI: 10.1002/elps.1150191103. View

Luconi M, Forti G, Baldi E . Pathophysiology of sperm motility. Front Biosci. 2005; 11:1433-47. DOI: 10.2741/1894. View

10.

Bohring C, Krause W . The characterization of human spermatozoa membrane proteins--surface antigens and immunological infertility. Electrophoresis. 1999; 20(4-5):971-6. DOI: 10.1002/(SICI)1522-2683(19990101)20:4/5<971::AID-ELPS971>3.0.CO;2-6. View

11.

Baker M, Witherdin R, Hetherington L, Cunningham-Smith K, Aitken R . Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis. Proteomics. 2005; 5(4):1003-12. DOI: 10.1002/pmic.200401100. View

12.

Comhaire F, Mahmoud A, Depuydt C, Zalata A, Christophe A . Mechanisms and effects of male genital tract infection on sperm quality and fertilizing potential: the andrologist's viewpoint. Hum Reprod Update. 1999; 5(5):393-8. DOI: 10.1093/humupd/5.5.393. View

13.

Ursini F, Heim S, Kiess M, Maiorino M, Roveri A, Wissing J . Dual function of the selenoprotein PHGPx during sperm maturation. Science. 1999; 285(5432):1393-6. DOI: 10.1126/science.285.5432.1393. View

14.

Moretti E, Scapigliati G, Pascarelli N, Baccetti B, Collodel G . Localization of AKAP4 and tubulin proteins in sperm with reduced motility. Asian J Androl. 2007; 9(5):641-9. DOI: 10.1111/j.1745-7262.2007.00267.x. View

15.

Petersen C, Fuzesi L, Hoyer-Fender S . Outer dense fibre proteins from human sperm tail: molecular cloning and expression analyses of two cDNA transcripts encoding proteins of approximately 70 kDa. Mol Hum Reprod. 1999; 5(7):627-35. DOI: 10.1093/molehr/5.7.627. View

16.

Wu T, Chu D . Sperm chromatin: fertile grounds for proteomic discovery of clinical tools. Mol Cell Proteomics. 2008; 7(10):1876-86. PMC: 2559940. DOI: 10.1074/mcp.R800005-MCP200. View

17.

Marin-Briggiler C, Gonzalez-Echeverria M, Munuce M, Ghersevich S, Caille A, Hellman U . Glucose-regulated protein 78 (Grp78/BiP) is secreted by human oviduct epithelial cells and the recombinant protein modulates sperm-zona pellucida binding. Fertil Steril. 2009; 93(5):1574-84. DOI: 10.1016/j.fertnstert.2008.12.132. View

18.

Brugh 3rd V, Lipshultz L . Male factor infertility: evaluation and management. Med Clin North Am. 2004; 88(2):367-85. DOI: 10.1016/S0025-7125(03)00150-0. View

19.

Infante J, Huszagh V . Synthesis of highly unsaturated phosphatidylcholines in the development of sperm motility: a role for epididymal glycerol-3-phosphorylcholine. Mol Cell Biochem. 1985; 69(1):3-6. DOI: 10.1007/BF00225921. View

20.

Secciani F, Bianchi L, Ermini L, Cianti R, Armini A, La Sala G . Protein profile of capacitated versus ejaculated human sperm. J Proteome Res. 2009; 8(7):3377-89. DOI: 10.1021/pr900031r. View