An Integrated Microfluidic Device for the Sorting of Yeast Cells Using Image Processing

Overview

Journal Sci Rep

Specialty Science

Date 2018 Feb 25

PMID 29476103

Citations 15

Authors

Bo Yang Yu

Caglar Elbuken

Chong Shen

Jan Paul Huissoon

Carolyn L Ren

Affiliations

Soon will be listed here.

Abstract

The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedious and inconsistent. This paper presents a microfluidic device integrated with microvalves for fluid control for the sorting of yeast cells using image processing algorithms and confirmation based on their fluorescent tag. The proposed device is completely automated, low cost and easy to implement in an academic research setting. Design details of the integrated microfluidic system are highlighted in this paper, along with experimental validation. Real time cell sorting was demonstrated with a cell detection rate of 12 cells per minute.

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