Expression of the V-src or V-fps Oncogene Increases Fructose 2,6-bisphosphate in Chick-embryo Fibroblasts. Novel Mechanism for the Stimulation of Glycolysis by Retroviruses

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Jun 1

PMID 2944513

Citations 16

Authors

L Bosca

M Mojena

J Ghysdael

G G Rousseau

L Hue

Affiliations

Soon will be listed here.

Abstract

The concentration of fructose 2,6-bisphosphate and the activity of 6-phosphofructo-2-kinase are increased after infection of chick-embryo fibroblasts with the Rous sarcoma virus, or with a temperature-sensitive mutant of this virus at the permissive, but not at the non-permissive, temperature. This is observed after transformation by retroviruses carrying either the v-src or v-fps, but not the v-mil and/or v-myc, oncogenes. Comparison of the effects of the Rous sarcoma virus with those of phorbol myristate acetate on fructose 2,6-bisphosphate suggests that both result from the stimulation of a step which is rate-limiting for 6-phosphofructo-2-kinase activation and which is also controlled by protein kinase C.

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References

Hatanaka M, Hanafusa H . Analysis of a functional change in membrane in the process of cell transformation by Rous sarcoma virus; alteration in the characteristics of sugar transport. Virology. 1970; 41(4):647-52. DOI: 10.1016/0042-6822(70)90429-0. View

Hanafusa H . Rapid transformation of cells by Rous sarcoma virus. Proc Natl Acad Sci U S A. 1969; 63(2):318-25. PMC: 223567. DOI: 10.1073/pnas.63.2.318. View

Singh V, Singh M, August J, HORECKER B . Alterations in glucose metabolism in chick-embryo cells transformed by Rous sarcoma virus: intracellular levels of glycolytic intermediates. Proc Natl Acad Sci U S A. 1974; 71(10):4129-32. PMC: 434342. DOI: 10.1073/pnas.71.10.4129. View

WEINHOUSE S . The Warburg hypothesis fifty years later. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1976; 87(2):115-26. DOI: 10.1007/BF00284370. View

Driedger P, Blumberg P . The effect of phorbol diesters on chicken embryo fibroblasts. Cancer Res. 1977; 37(9):3257-65. View

Diamond I, Legg A, Schneider J, Rozengurt E . Glycolysis in quiescent cultures of 3T3 cells. Stimulation by serum, epidermal growth factor, and insulin in intact cells and persistence of the stimulation after cell homogenization. J Biol Chem. 1978; 253(3):866-71. View

Collett M, Erikson R . Protein kinase activity associated with the avian sarcoma virus src gene product. Proc Natl Acad Sci U S A. 1978; 75(4):2021-4. PMC: 392475. DOI: 10.1073/pnas.75.4.2021. View

Royer-Pokora B, Beug H, Claviez M, Winkhardt H, Friis R, Graf T . Transformation parameters in chicken fibroblasts transformed by AEV and MC29 avian leukemia viruses. Cell. 1978; 13(4):751-60. DOI: 10.1016/0092-8674(78)90225-8. View

Hunter E . Biological techniques for avian sarcoma viruses. Methods Enzymol. 1979; 58:379-93. DOI: 10.1016/s0076-6879(79)58153-1. View

10.

OBrien T, Saladik D, Diamond L . The tumor promoter 12-O-tetradecanoylphorbol-13-acetate stimulates lactate production in BALB/c 3T3 preadipose cells. Biochem Biophys Res Commun. 1979; 88(1):103-10. DOI: 10.1016/0006-291x(79)91702-9. View

11.

Pouyssegur J, Franchi A, Silvestre P . Relationship between increased aerobic glycolysis and DNA synthesis initiation studied using glycolytic mutant fibroblasts. Nature. 1980; 287(5781):445-7. DOI: 10.1038/287445a0. View

12.

Van Schaftingen E, Hue L, HERS H . Fructose 2,6-bisphosphate, the probably structure of the glucose- and glucagon-sensitive stimulator of phosphofructokinase. Biochem J. 1980; 192(3):897-901. PMC: 1162415. DOI: 10.1042/bj1920897. View

13.

Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U, NISHIZUKA Y . Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982; 257(13):7847-51. View

14.

Cooper J, Reiss N, Schwartz R, Hunter T . Three glycolytic enzymes are phosphorylated at tyrosine in cells transformed by Rous sarcoma virus. Nature. 1983; 302(5905):218-23. DOI: 10.1038/302218a0. View

15.

Bruni P, Farnararo M, Vasta V, DAlessandro A . Increase of the glycolytic rate in human resting fibroblasts following serum stimulation. The possible role of the fructose-2,6-bisphosphate. FEBS Lett. 1983; 159(1-2):39-42. DOI: 10.1016/0014-5793(83)80412-8. View

16.

Weber M, Nakamura K, Salter D . Molecular events leading to enhanced glucose transport in Rous sarcoma virus-transformed cells. Fed Proc. 1984; 43(8):2246-50. View

17.

NISHIZUKA Y . The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature. 1984; 308(5961):693-8. DOI: 10.1038/308693a0. View

18.

Sugimoto Y, Whitman M, Cantley L, Erikson R . Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol. Proc Natl Acad Sci U S A. 1984; 81(7):2117-21. PMC: 345448. DOI: 10.1073/pnas.81.7.2117. View

19.

Hunter T . The proteins of oncogenes. Sci Am. 1984; 251(2):70-9. DOI: 10.1038/scientificamerican0884-70. View

20.

Moelling K, Heimann B, Beimling P, Rapp U, Sander T . Serine- and threonine-specific protein kinase activities of purified gag-mil and gag-raf proteins. Nature. 1984; 312(5994):558-61. DOI: 10.1038/312558a0. View