Adenovirus E1B Proteins Are Required for Accumulation of Late Viral MRNA and for Effects on Cellular MRNA Translation and Transport

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1985 Oct 1

PMID 2942759

Citations 112

Authors

L E Babiss

H S Ginsberg

J E Darnell Jr

Affiliations

Soon will be listed here.

Abstract

Late in adenovirus infection, large amounts of viral mRNA accumulate while cell mRNA transport and translation decrease. Viruses deleted in the E1B region of type 5 adenovirus do not produce the same outcome: (i) viral mRNA synthesis by the mutants is normal, delivery to the cytoplasm is 50 to 75% of normal, but steady-state levels of viral mRNA are decreased 10-fold; (ii) cell mRNA synthesis and transport continue normally in the mutant virus-infected cell; and (iii) translation of preexisting cell mRNA which is disrupted in wild-type infection remains normal in mutant-virus-infected cells. Thus E1B proteins are required for accumulation of virus mRNA and for induction of the failure of host cell mRNA transport and translation. If a single function is involved, by inference the transport and some aspect of translation of mRNAs could be linked.

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