In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. Pombe

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2018 Feb 10

PMID 29423855

Citations 2

Authors

Jinpu Yang

Fei Li

Affiliations

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Abstract

Chromatin-associated proteins play critical roles in many cellular processes, including gene expression, epigenetic regulation, DNA repair, recombination, and replication. Especially, epigenetic landscape, shaped by a variety of chromatin-binding proteins, is dynamic and regulated in a context-dependent manner. In situ chromatin-binding assay is a powerful but simple tool to investigate how proteins, such as epigenetic components, associate with chromatin. This approach relies on the fact that chromatin bound proteins are more resistant to detergent extraction. Here, we describe a protocol for the in situ chromatin-binding assay used in Schizosaccaromyces pombe.

Citing Articles

Cell Cycle-Regulated Transcription of CENP-A by the MBF Complex Ensures Optimal Level of CENP-A for Centromere Formation.

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Heterochromatin and RNAi regulate centromeres by protecting CENP-A from ubiquitin-mediated degradation.

Yang J, Sun S, Zhang S, Gonzalez M, Dong Q, Chi Z PLoS Genet. 2018; 14(8):e1007572.

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