» Articles » PMID: 29412907

Investigation on Antioxidant, Angiotensin Converting Enzyme and Dipeptidyl Peptidase IV Inhibitory Activity of Bambara Bean Protein Hydrolysates

Overview
Journal Food Chem
Date 2018 Feb 8
PMID 29412907
Citations 19
Authors
Affiliations
Soon will be listed here.
Abstract

Protein isolate was hydrolysed by Alcalase, thermolysin and trypsin. BBPH produced by Alcalase showed highest angiotensin-converting enzyme (ACE) inhibitory properties (IC: 52 µg/mL). Hydrolysates produced by Alcalase and thermolysin exhibited similar dipeptidyl peptidase-IV (DPP-IV) inhibitory activity (IC: 1.73 mg/mL), while low inhibitory activity was observed for hydrolysate produced by trypsin. BBPH also showed protective effect against oxidative stress with significant 2,2-diphenyl-1-picrylhydrazyl radical scavenging and ferrous chelating activity. Bioactive peptides of BBPH produced by thermolysin showed better resistance to simulated gastrointestinal digestion (SGID), while the DPP-IV and ACE inhibitory properties were significantly reduced. Molecular weight distribution showed significant reduction in peptides of the molecular weight range 200-400 Da in BBPH produced by Alcalase, after SGID. LC-ESI-TOF-MS and in silico analysis showed the presence of potential peptides with both ACE and DPP-IV inhibitory properties in BBPH produced by thermolysin.

Citing Articles

TRPA1-Activated Peptides from Saiga Antelope Horn: Screening, Interaction Mechanism, and Bioactivity.

Wang C, Wu C, Song L Int J Mol Sci. 2025; 26(5).

PMID: 40076741 PMC: 11900222. DOI: 10.3390/ijms26052119.


Computational Screening for the Dipeptidyl Peptidase-IV Inhibitory Peptides from Putative Hemp Seed Hydrolyzed Peptidome as a Potential Antidiabetic Agent.

Thongtak A, Yutisayanuwat K, Harnkit N, Noikaew T, Chumnanpuen P Int J Mol Sci. 2024; 25(11).

PMID: 38891918 PMC: 11171819. DOI: 10.3390/ijms25115730.


The first bioactive (angiotensin-converting enzyme-inhibitory) peptide isolated from pearl matrix protein.

Wu C, Yin Z, Wang Y, Chen X, Li B, Wang Q Heliyon. 2024; 10(7):e28060.

PMID: 38560194 PMC: 10979060. DOI: 10.1016/j.heliyon.2024.e28060.


Protein hydrolysates of seed: Antioxidant and antihyperglycaemic potential as ingredient for the management of type-2 diabetes.

Bakwo Bassogog C, Nyobe C, Sabine F, Dupon A, Ngui S, Minka S Heliyon. 2024; 10(7):e28368.

PMID: 38560105 PMC: 10981051. DOI: 10.1016/j.heliyon.2024.e28368.


Identification of the Bioavailable Peptidome of Chia Protein Hydrolysate and the In Silico Evaluation of Its Antioxidant and ACE Inhibitory Potential.

Villanueva A, Rivero-Pino F, Martin M, Gonzalez-de la Rosa T, Montserrat-de la Paz S, Millan-Linares M J Agric Food Chem. 2024; 72(6):3189-3199.

PMID: 38305180 PMC: 10870759. DOI: 10.1021/acs.jafc.3c05331.