Preparative Separation and Purification of Deoxyschizandrin from Schisandrae Sphenantherae Fructus by High-speed Counter-current Chromatography

Overview

Journal J Pharm Anal

Specialty Chemistry

Date 2018 Feb 7

PMID 29403850

Citations 3

Authors

Xin-Ying Li

Mei Yang

Jie-Yun Huang

Xiao-Xue Yu

Min-Qian Zhao

Zhi-Kun Liang

Zhi-Sheng Xie

Xin-Jun Xu

Affiliations

Soon will be listed here.

Abstract

A high-speed counter-current chromatography (HSCCC) method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step. The purity of deoxyschizandrin was 98.5%, and the structure was identified by MS, UV and NMR. This method was simple, fast, convenient and appropriate to prepare pure compound as reference substances for related research on Schisandrae Sphenantherae Fructus.

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References

Peng J, Fan G, Qu L, Zhou X, Wu Y . Application of preparative high-speed counter-current chromatography for isolation and separation of schizandrin and gomisin A from Schisandra chinensis. J Chromatogr A. 2005; 1082(2):203-7. DOI: 10.1016/j.chroma.2005.05.091. View

Huyke C, Engel K, Simon-Haarhaus B, Quirin K, Schempp C . Composition and biological activity of different extracts from Schisandra sphenanthera and Schisandra chinensis. Planta Med. 2007; 73(10):1116-26. DOI: 10.1055/s-2007-981559. View

Lu Y, Chen D . Analysis of Schisandra chinensis and Schisandra sphenanthera. J Chromatogr A. 2008; 1216(11):1980-90. DOI: 10.1016/j.chroma.2008.09.070. View

Liu C, Fang S, Huang M, Kao Y, Hsu J . Studies on the active principles of Schisandra sphenanthera Rehd. et Wils. The structures of schisantherin A, B, C, D, E, and the related compounds. Sci Sin. 1978; 21(4):483-502. View