Rapid Classification and Identification of Chemical Components of Schisandra Chinensis by UPLC-Q-TOF/MS Combined with Data Post-Processing

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2017 Oct 21

PMID 29053630

Citations 21

Authors

Shenshen Yang

Lanlan Shan

Houmin Luo

Xue Sheng

Jun Du

Yubo Li

Affiliations

Soon will be listed here.

Abstract

(known in Chinese as WuWeiZi, WWZ) has observable effects such as astringing the lung to stop coughs, arresting sweating, preserving semen and preventing diarrhea. The major components of WWZ include lignans, triterpenoids, organic acids and fatty acids. In this paper, a reliable method for the rapid identification of multiple components in WWZ by their characteristic fragments and neutral losses using UPLC-Q-TOF/MS technology was developed. After review of the literature and some reference experiments, the fragmentation pattern of several compounds were studied and summarized. Then, according to the corresponding characteristic fragments coupled with neutral losses in the positive or negative ion mode produced by different types of substances a rapid identification of target compounds was achieved. Finally, a total of 30 constituents of WWZ were successfully identified, including 15 lignans, nine triterpenoids, three organic acids and three fatty acids. The method established in this study not only provides a comprehensive analysis of the chemical ingredients of WWZ, providing a basis for further phytochemical studies on WWZ but also provides a more efficient way to solve the problem of identification of complex chemical constituents in traditional Chinese medicines.

Citing Articles

Molecular dynamics simulation-driven focused virtual screening and experimental validation of Fisetin as an inhibitor of Helicobacter pylori HtrA protease.

Gao L, Jiang X, Duan H, Shen Y, Gu K, Huang K Mol Divers. 2025; .

PMID: 39988708 DOI: 10.1007/s11030-025-11137-2.

A Review of the Biological Activity and Structure-Property Relationships of the Main Compounds from .

Skalski B, Kuzniak E, Kowalska I, Sikora M, Olas B Nutrients. 2025; 17(3).

PMID: 39940298 PMC: 11821066. DOI: 10.3390/nu17030436.

Rapid identification of chemical profiles and of Huan Shao Dan and potential anti-aging metabolites by high-resolution mass spectrometry, sequential metabolism, and deep learning model.

Li X, Pan F, Wang L, Zhang J, Wang X, Qi D Front Pharmacol. 2024; 15:1432592.

PMID: 39474607 PMC: 11518704. DOI: 10.3389/fphar.2024.1432592.

Evaluating the Root Extract of (Nakai) Moldenke: An Analysis of Active Constituents, Antioxidant Potential, and Investigation of Hepatoprotective Effects in Rats.

Xing Z, Han Y, Pang H, Li L, Xia G, Zhu J Molecules. 2024; 29(19).

PMID: 39407630 PMC: 11478139. DOI: 10.3390/molecules29194701.

[Identification of chemical components of large-leaf yellow tea by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry].

Huang R, Rong X, Fu X, Chen C, Chu J, Xu N Se Pu. 2024; 42(9):837-855.

PMID: 39198943 PMC: 11358872. DOI: 10.3724/SP.J.1123.2023.10021.

References

Gao L, Guo T, Xu X, Yang J . Rapid identification and simultaneous analysis of multiple constituents from Rheum tanguticum Maxim. ex Balf. by UPLC/Q-TOF-MS. Nat Prod Res. 2017; 31(13):1529-1535. DOI: 10.1080/14786419.2017.1280491. View

Zhitnitsky D, Rose J, Lewinson O . The highly synergistic, broad spectrum, antibacterial activity of organic acids and transition metals. Sci Rep. 2017; 7:44554. PMC: 5353632. DOI: 10.1038/srep44554. View

Zhou Y, Huang S, Pu J, Li J, Ding L, Chen D . Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometric procedure for qualitative and quantitative analyses of nortriterpenoids and lignans in the genus Schisandra. J Pharm Biomed Anal. 2011; 56(5):916-27. DOI: 10.1016/j.jpba.2011.07.045. View

Shi P, He Q, Zhang Y, Qu H, Cheng Y . Characterisation and identification of isomeric dibenzocyclooctadiene lignans from Schisandra Chinensis by high-performance liquid chromatography combined with electrospray ionisation tandem mass spectrometry. Phytochem Anal. 2009; 20(3):197-206. DOI: 10.1002/pca.1115. View

Liu M, Zhao S, Wang Z, Wang Y, Liu T, Li S . Identification of metabolites of deoxyschizandrin in rats by UPLC-Q-TOF-MS/MS based on multiple mass defect filter data acquisition and multiple data processing techniques. J Chromatogr B Analyt Technol Biomed Life Sci. 2014; 949-950:115-26. DOI: 10.1016/j.jchromb.2013.12.022. View

Tak V, Purohit A, Pardasani D, Goud D, Jain R, Dubey D . Simultaneous detection and identification of precursors, degradation and co-products of chemical warfare agents in drinking water by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry. J Chromatogr A. 2014; 1370:80-92. DOI: 10.1016/j.chroma.2014.10.030. View

Zhu L, Li B, Liu X, Meng X . Purification of two triterpenoids from Schisandra chinensis by macroporous resin combined with high-speed counter-current chromatography. J Chromatogr Sci. 2013; 52(9):1082-8. DOI: 10.1093/chromsci/bmt168. View

Chu C, Zhang S, Tong S, Li X, Yan J . An efficient strategy for the extraction and purification of lignans from Schisandra chinensis by a combination of supercritical fluid extraction and high-speed counter-current chromatography. J Sep Sci. 2013; 36(24):3958-64. DOI: 10.1002/jssc.201300896. View

Li X, Yang M, Huang J, Yu X, Zhao M, Liang Z . Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography. J Pharm Anal. 2018; 3(6):429-433. PMC: 5761001. DOI: 10.1016/j.jpha.2013.07.002. View

10.

Liu G, Zhao Y, Li Y, Wang X, Si H, Huang W . Qualitative and quantitative analysis of major constituents from Dazhu Hongjingtian capsule by UPLC/Q-TOF-MS/MS combined with UPLC/QQQ-MS/MS. Biomed Chromatogr. 2016; 31(6). DOI: 10.1002/bmc.3887. View

11.

Huang C, Xu Q, Chen C, Song C, Xu Y, Xiang Y . The rapid discovery and identification of physalins in the calyx of Physalis alkekengi L.var.franchetii (Mast.) Makino using ultra-high performance liquid chromatography-quadrupole time of flight tandem mass spectrometry together with a novel.... J Chromatogr A. 2014; 1361:139-52. DOI: 10.1016/j.chroma.2014.08.004. View

12.

Lou Z, Zhang H, Gong C, Zhu Z, Zhao L, Xu Y . Analysis of lignans in Schisandra chinensis and rat plasma by high-performance liquid chromatography diode-array detection, time-of-flight mass spectrometry and quadrupole ion trap mass spectrometry. Rapid Commun Mass Spectrom. 2009; 23(6):831-42. DOI: 10.1002/rcm.3931. View

13.

Zhang J, Zhang Q, Li N, Wang Z, Lu J, Qiao Y . Diagnostic fragment-ion-based and extension strategy coupled to DFIs intensity analysis for identification of chlorogenic acids isomers in Flos Lonicerae Japonicae by HPLC-ESI-MS(n). Talanta. 2013; 104:1-9. DOI: 10.1016/j.talanta.2012.11.012. View

14.

Sterbova H, Sevcikova P, Kvasnickova L, Glatz Z, Slanina J . Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography. Electrophoresis. 2002; 23(2):253-8. DOI: 10.1002/1522-2683(200202)23:2<253::AID-ELPS253>3.0.CO;2-H. View

15.

Xia Y, Yang B, Liang J, Yang Q, Wang D, Kuang H . Quantitative analysis and fingerprint profiles for quality control of Fructus Schisandrae by gas chromatography: mass spectrometry. ScientificWorldJournal. 2014; 2014:806759. PMC: 3913529. DOI: 10.1155/2014/806759. View

16.

Jiang P, Lu Y, Chen D . Authentication of Schisandra chinensis and Schisandra sphenanthera in Chinese patent medicines. J Pharm Biomed Anal. 2016; 131:263-271. DOI: 10.1016/j.jpba.2016.08.040. View

17.

Xu L, Mu L, Peng J, Liu W, Tan X, Li Z . UPLC-Q-TOF-MS(E) analysis of the constituents of Ding-Zhi-Xiao-Wan, a traditional Chinese antidepressant, in normal and depressive rats. J Chromatogr B Analyt Technol Biomed Life Sci. 2015; 1026:36-42. DOI: 10.1016/j.jchromb.2015.07.043. View

18.

Huang X, Song F, Liu Z, Liu S . Studies on lignan constituents from Schisandra chinensis (Turcz.) Baill. fruits using high-performance liquid chromatography/electrospray ionization multiple-stage tandem mass spectrometry. J Mass Spectrom. 2007; 42(9):1148-61. DOI: 10.1002/jms.1246. View

19.

Song X, Li Y, Shi Y, Jin L, Chen J . Quality control of traditional Chinese medicines: a review. Chin J Nat Med. 2013; 11(6):596-607. DOI: 10.1016/S1875-5364(13)60069-2. View

20.

Liu H, Lai H, Jia X, Liu J, Zhang Z, Qi Y . Comprehensive chemical analysis of Schisandra chinensis by HPLC-DAD-MS combined with chemometrics. Phytomedicine. 2013; 20(12):1135-43. DOI: 10.1016/j.phymed.2013.05.001. View