Comprehensive Characterization of Chorionic Villi-derived Mesenchymal Stromal Cells from Human Placenta

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2018 Feb 7

PMID 29402304

Citations 25

Authors

Monica S Ventura Ferreira

Michaela Bienert

Katrin Muller

Bjorn Rath

Tamme Goecke

Christian Oplander

Till Braunschweig

Petra Mela

Tim H Brummendorf

Fabian Beier

Sabine Neuss

Affiliations

Soon will be listed here.

Abstract

Background: Studies in which mesenchymal stromal cells (MSC) from the placenta are compared with multiple MSC types from other sources are rare. The chorionic plate of the human placenta is mainly composed of fetal blood vessels embedded in fetal stroma tissue, lined by trophoblastic cells and organized into chorionic villi (CV) structures.

Methods: We comprehensively characterized human MSC collected from postnatal human chorionic villi of placenta (CV-MSC) by analyzing their growth and proliferation potential, differentiation, immunophenotype, extracellular matrix production, telomere length, aging phenotype, and plasticity.

Results: Immunophenotypic characterization of CV-MSC confirmed the typical MSC marker expression as defined by the International Society for Cellular Therapy. The surface marker profile was consistent with increased potential for proliferation, vascular localization, and early myogenic marker expression. CV-MSC retained multilineage differentiation potential and extracellular matrix remodeling properties. They have undergone reduced telomere loss and delayed onset of cellular senescence as they aged in vitro compared to three other MSC sources. We present evidence that increased human telomerase reverse transcriptase gene expression could not explain the exceptional telomere maintenance and senescence onset delay in cultured CV-MSC. Our in-vitro tumorigenesis detection assay suggests that CV-MSC are not prone to undergo malignant transformation during long-term in-vitro culture. Besides SOX2 expression, no other pluripotency features were observed in early and late passages of CV-MSC.

Conclusions: Our work brings forward two remarkable characteristics of CV-MSC, the first being their extended life span as a result of delayed replicative senescence and the second being a delayed aged phenotype characterized by improved telomere length maintenance. MSC from human placenta are very attractive candidates for stem cell-based therapy applications.

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References

Ferreira M, Labude N, Piroth D, Jahnen-Dechent W, Knuchel R, Hieronymus T . Compatibility of different polymers for cord blood-derived hematopoietic progenitor cells. J Mater Sci Mater Med. 2011; 23(1):109-16. DOI: 10.1007/s10856-011-4483-4. View

Gonzalo S, Jaco I, Fraga M, Chen T, Li E, Esteller M . DNA methyltransferases control telomere length and telomere recombination in mammalian cells. Nat Cell Biol. 2006; 8(4):416-24. DOI: 10.1038/ncb1386. View

Yu J, Su X, Zhu C, Pan Q, Yang J, Ma J . GFP Labeling and Hepatic Differentiation Potential of Human Placenta-Derived Mesenchymal Stem Cells. Cell Physiol Biochem. 2015; 35(6):2299-308. DOI: 10.1159/000374033. View

Fehrer C, Lepperdinger G . Mesenchymal stem cell aging. Exp Gerontol. 2005; 40(12):926-30. DOI: 10.1016/j.exger.2005.07.006. View

Avilion A, Nicolis S, Pevny L, Perez L, Vivian N, Lovell-Badge R . Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev. 2003; 17(1):126-40. PMC: 195970. DOI: 10.1101/gad.224503. View

Ilancheran S, Moodley Y, Manuelpillai U . Human fetal membranes: a source of stem cells for tissue regeneration and repair?. Placenta. 2008; 30(1):2-10. DOI: 10.1016/j.placenta.2008.09.009. View

Shin K, Na K, Lee H, Kim D, Shin S, Kim J . Characterization of Fetal Tissue-derived Mesenchymal Stem Cells. Int J Stem Cells. 2014; 2(1):51-8. PMC: 4021794. DOI: 10.15283/ijsc.2009.2.1.51. View

Barlow S, Brooke G, Chatterjee K, Price G, Pelekanos R, Rossetti T . Comparison of human placenta- and bone marrow-derived multipotent mesenchymal stem cells. Stem Cells Dev. 2008; 17(6):1095-107. DOI: 10.1089/scd.2007.0154. View

Okolicsanyi R, Camilleri E, Oikari L, Yu C, Cool S, van Wijnen A . Human Mesenchymal Stem Cells Retain Multilineage Differentiation Capacity Including Neural Marker Expression after Extended In Vitro Expansion. PLoS One. 2015; 10(9):e0137255. PMC: 4565666. DOI: 10.1371/journal.pone.0137255. View

10.

Hackett J, Surani M . Regulatory principles of pluripotency: from the ground state up. Cell Stem Cell. 2014; 15(4):416-430. DOI: 10.1016/j.stem.2014.09.015. View

11.

Castrechini N, Murthi P, Gude N, Erwich J, Gronthos S, Zannettino A . Mesenchymal stem cells in human placental chorionic villi reside in a vascular Niche. Placenta. 2010; 31(3):203-12. DOI: 10.1016/j.placenta.2009.12.006. View

12.

Rus Ciuca D, Soritau O, Susman S, Pop V, Mihu C . Isolation and characterization of chorionic mesenchyal stem cells from the placenta. Rom J Morphol Embryol. 2011; 52(3):803-8. View

13.

Ryan J, Barry F, Murphy J, Mahon B . Mesenchymal stem cells avoid allogeneic rejection. J Inflamm (Lond). 2005; 2:8. PMC: 1215510. DOI: 10.1186/1476-9255-2-8. View

14.

Silva J, Nichols J, Theunissen T, Guo G, van Oosten A, Barrandon O . Nanog is the gateway to the pluripotent ground state. Cell. 2009; 138(4):722-37. PMC: 3437554. DOI: 10.1016/j.cell.2009.07.039. View

15.

Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K . Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat Cell Biol. 2007; 9(6):625-35. DOI: 10.1038/ncb1589. View

16.

Sabapathy V, Ravi S, Srivastava V, Srivastava A, Kumar S . Long-term cultured human term placenta-derived mesenchymal stem cells of maternal origin displays plasticity. Stem Cells Int. 2012; 2012:174328. PMC: 3329664. DOI: 10.1155/2012/174328. View

17.

Blasco M . Telomeres and human disease: ageing, cancer and beyond. Nat Rev Genet. 2005; 6(8):611-22. DOI: 10.1038/nrg1656. View

18.

Portmann-Lanz C, Schoeberlein A, Huber A, Sager R, Malek A, Holzgreve W . Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration. Am J Obstet Gynecol. 2006; 194(3):664-73. DOI: 10.1016/j.ajog.2006.01.101. View

19.

Qin S, Kusuma G, Al-Sowayan B, Pace R, Isenmann S, Pertile M . Establishment and characterization of fetal and maternal mesenchymal stem/stromal cell lines from the human term placenta. Placenta. 2016; 39:134-46. DOI: 10.1016/j.placenta.2016.01.018. View

20.

Chambers I, Silva J, Colby D, Nichols J, Nijmeijer B, Robertson M . Nanog safeguards pluripotency and mediates germline development. Nature. 2007; 450(7173):1230-4. DOI: 10.1038/nature06403. View