Diagnostic Validity of Flow Cytometry Vs Manual Counting of Polymorphonuclear Leukocytes in Spontaneous Bacterial Peritonitis

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2018 Feb 1

PMID 29383775

Citations 3

Authors

Heba Ahmed Osman

Sanaa Shaker Aly

Eman M Salah-Eldin

Muhammad Abbas El-Masry

Mohammed H Hassan

Affiliations

Soon will be listed here.

Abstract

Background: Spontaneous bacterial peritonitis (SBP) is frequently occurring infection among patients with liver cirrhosis, defined by polymorphonuclear (PMN) leukocytic count ≥250 cell/mm with or without a positive ascitic fluid (AF) bacterial culture. So, this study aimed to investigate the diagnostic value of flow cytometry versus manual counting of ascitic fluid PMNL in cirrhotic patients, with clinical suspicion of SBP.

Methods: A hospital-based cross-sectional study was carried out on 320 cirrhotic patients with clinical suspicion of SBP. Abdominal paracentesis was performed in all cases for microscopic manual and flow cytometry counting of PMNL. Anti-HLA-DR, anti-CD15, anti-CD16, and anti-CD45 monoclonal antibodies were used for flow cytometry method.

Results: Flow cytometric PMNL count had 100% sensitivity and specificity, while manual PMNL count had a sensitivity of 65.52% and specificity of 90% with significant difference (P value < .05).

Conclusion: Flow cytometry is more reliable rapid method for PMNL counting, than the manual method that is less accurate and time-consuming in diagnosing clinically suspected SBP.

Citing Articles

Diagnostic accuracy of mean platelet volume in cirrhotic patients with spontaneous bacterial peritonitis presented to the emergency department.

Naeimi Bafghi N, Torabi M, Ashkar F, Mirzaee M Caspian J Intern Med. 2024; 16(1):151-157.

PMID: 39619750 PMC: 11607127. DOI: 10.22088/cjim.16.1.151.

Ascitic fluid polymorphic nuclear cell count impacts on outcome of cirrhotic patients with ascites.

Simbrunner B, Rothenbacher A, Haslacher H, Bauer D, Chromy D, Bucsics T United European Gastroenterol J. 2019; 7(5):651-661.

PMID: 31210943 PMC: 6545717. DOI: 10.1177/2050640619843000.

Diagnostic validity of flow cytometry vs manual counting of polymorphonuclear leukocytes in spontaneous bacterial peritonitis.

Osman H, Aly S, Salah-Eldin E, El-Masry M, Hassan M J Clin Lab Anal. 2018; 32(5):e22395.

PMID: 29383775 PMC: 6816940. DOI: 10.1002/jcla.22395.

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