Microarray Analysis of Lung Long Non-coding RNAs in Cigarette Smoke-exposed Mouse Model

Overview

Journal Oncotarget

Specialty Oncology

Date 2018 Feb 1

PMID 29383188

Citations 8

Authors

Hao Wang

Lei Chen

Diandian Li

Ni Zeng

Yanqiu Wu

Tao Wang

Yongchun Shen

Dan Xu

Fuqiang Wen

Affiliations

Soon will be listed here.

Abstract

Several studies have demonstrated the function of long nonâ€'coding RNAs (lncRNAs) in various biological processes, yet their role underlying the susceptibility to cigarette smoke (CS)-induced airway inflammation remains limited. In the present study, we aimed to profile the expression of lncRNAs and mRNAs in CS-exposed mice. C57BL/6 mice were assigned into a single cigarette-smoking machine with or without CS exposure for 4 weeks, followed by lung tissue harvest and RNA isolation. Microarray analysis identified 108 lncRNAs and 119 mRNAs with differential expression levels in CS-exposed mouse lung tissue compared with those in control mice. The expression patterns of several lncRNAs were further confirmed by qRT-PCR. GO and pathway analyses showed that the altered mRNAs were mainly related to the processes of immune response, defense response and cell chemotaxis, cytokine-cytokine receptor interaction and chemokine signaling pathway. Moreover, a single lncRNA may co-expressed with several mRNAs, and so was the mRNA. Our findings uncovered the expression profile of lncRNAs and mRNAs in the lungs of CS-exposed mice, which may offer new insights into pathogenesis of CS-associated airway inflammatory disorders.

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