Changing Expression Profiles of LncRNAs, MRNAs, CircRNAs and MiRNAs During Osteoclastogenesis

Overview

Journal Sci Rep

Specialty Science

Date 2016 Feb 10

PMID 26856880

Citations 91

Authors

Ce Dou

Zhen Cao

Bo Yang

Ning Ding

Tianyong Hou

Fei Luo

Fei Kang

Jianmei Li

Xiaochao Yang

Hong Jiang

Junyu Xiang

Hongyu Quan

Jianzhong Xu

Shiwu Dong

Affiliations

Soon will be listed here.

Abstract

Bone is a dynamic organ continuously undergoing shaping, repairing and remodeling. The homeostasis of bone is maintained by the balance between osteoblastic bone formation and osteoclastic bone resorption. Osteoclasts (OCs) are specialized multinucleated cells derived from hematopoietic stem cells (HSCs) or monocytes/macrophage progenitor cells. There are different stages during osteoclastogenesis, and one of the most important steps to form functional osteoclasts is realized by cell-cell fusion. In our study, microarray was performed to detect the expression profiles of lncRNA, mRNA, circRNA and miRNA at different stages during osteoclastogenesis of RAW264.7 cells. Often changed RNAs were selected and clustered among the four groups with Venn analysis. The results revealed that expressions of 518 lncRNAs, 207 mRNAs, 24 circRNAs and 37 miRNAs were often altered at each stage during OC differentiation. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analysis were performed to predict the functions of differentially expressed lncRNAs and co-expressed potential targeting genes. Co-expression networks of lncRNA-mRNA and circRNA-miRNA were constructed based on the correlation analysis between the differentially expressed RNAs. The present study provided a systematic perspective on the potential function of non-coding RNAs (ncRNAs) during osteoclastogenesis.

Citing Articles

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